QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (34) Citing Articles via Google Scholar Google Scholar Articles by Nishimura, W. Articles by Hata, Y. Search for Related Content PubMed PubMed Citation Articles by Nishimura, W. Articles by Hata, Y.

 Previous Article  |  Next Article 

The Journal of Neuroscience, February 1, 2002, 22(3):757-765

Interaction of Synaptic Scaffolding Molecule and -Catenin

Wataru Nishimura^{1, 2}, Ikuko Yao¹, Junko Iida¹, Noriaki Tanaka², and Yutaka Hata¹

¹ Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8519, Japan, and ² First Department of Surgery, Okayama University Medical School, Okayama 700-8558, Japan

Synaptic scaffolding molecule (S-SCAM) is a synaptic membrane-associated guanylate kinase with inverted domain organization (MAGI) that interacts with NMDA receptor subunits and neuroligin. In epithelial cells, the non-neuronal isoform of S-SCAM (MAGI-1) is localized at tight or adherens junctions. Recent studies have revealed that the polarized targeting of MAGI-1 to the lateral membrane is mediated by its C-terminal region and that MAGI-1 interacts with -catenin in epithelial cells. In this article, we report that S-SCAM interacts with -catenin in neurons. -Catenin is coimmunoprecipitated with S-SCAM from rat brain. Both S-SCAM and -catenin are localized at synapses and are partially colocalized. The C-terminal region of S-SCAM binds to the C-terminal region of -catenin. We have tested how the interaction between S-SCAM and -catenin plays a role in the synaptic targeting of S-SCAM and -catenin. S-SCAM is targeted to synapses via the C-terminal postsynaptic density-95/Dlg-A/ZO-1 (PDZ) domain. -Catenin is targeted to synapses with armadillo repeats. The overexpressed C-terminal region of -catenin blocks the synaptic targeting of S-SCAM. The overexpressed C-terminal region of S-SCAM is partially targeted to synapses and forms a small number of clusters. In the presence of overexpressed -catenin, the C-terminal region of S-SCAM forms more clusters at synapses. These data suggest that the synaptic targeting of S-SCAM is mediated by the interaction with -catenin.

Key words: -catenin; synaptic scaffolding molecule; membrane-associated guanylate kinase with inverted domain organization; NMDA receptor; armadillo repeats; PDZ domain

---

Copyright © 2002 Society for Neuroscience  0270-6474/02/223757-09$05.00/0

This article has been cited by other articles:

				T. Okuda, L. M. Y. Yu, L. A. Cingolani, R. Kemler, and Y. Goda beta-Catenin regulates excitatory postsynaptic strength at hippocampal synapses PNAS, August 14, 2007; 104(33): 13479 - 13484. [Abstract] [Full Text] [PDF]

				J. B. Silverman, S. Restituito, W. Lu, L. Lee-Edwards, L. Khatri, and E. B. Ziff Synaptic Anchorage of AMPA Receptors by Cadherins through Neural Plakophilin-Related Arm Protein AMPA Receptor-Binding Protein Complexes J. Neurosci., August 8, 2007; 27(32): 8505 - 8516. [Abstract] [Full Text] [PDF]

				R. A. Al-Hallaq, T. P. Conrads, T. D. Veenstra, and R. J. Wenthold NMDA Di-Heteromeric Receptor Populations and Associated Proteins in Rat Hippocampus J. Neurosci., August 1, 2007; 27(31): 8334 - 8343. [Abstract] [Full Text] [PDF]

				J. Iida, H. Ishizaki, M. Okamoto-Tanaka, A. Kawata, K. Sumita, S. Ohgake, Y. Sato, H. Yorifuji, N. Nukina, K. Ohashi, et al. Synaptic Scaffolding Molecule {alpha} Is a Scaffold To Mediate N-Methyl-D-Aspartate Receptor-Dependent RhoA Activation in Dendrites Mol. Cell. Biol., June 15, 2007; 27(12): 4388 - 4405. [Abstract] [Full Text] [PDF]

				Y. Zhang, S. Yeh, B. A. Appleton, H. A. Held, P. J. Kausalya, D. C. Y. Phua, W. Lee Wong, L. A. Lasky, C. Wiesmann, W. Hunziker, et al. Convergent and Divergent Ligand Specificity among PDZ Domains of the LAP and Zonula Occludens (ZO) Families J. Biol. Chem., August 4, 2006; 281(31): 22299 - 22311. [Abstract] [Full Text] [PDF]

				F. Deng, M. G. Price, C. F. Davis, M. Mori, and D. L. Burgess Stargazin and other transmembrane AMPA receptor regulating proteins interact with synaptic scaffolding protein MAGI-2 in brain. J. Neurosci., July 26, 2006; 26(30): 7875 - 7884. [Abstract] [Full Text] [PDF]

				A. Kawata, J. Iida, M. Ikeda, Y. Sato, H. Mori, A. Kansaku, K. Sumita, N. Fujiwara, C. Rokukawa, M. Hamano, et al. CIN85 Is Localized at Synapses and Forms a Complex with S-SCAM via Dendrin. J. Biochem., May 1, 2006; 139(5): 931 - 939. [Abstract] [Full Text] [PDF]

				S. Lehtonen, J. J. Ryan, K. Kudlicka, N. Iino, H. Zhou, and M. G. Farquhar Cell junction-associated proteins IQGAP1, MAGI-2, CASK, spectrins, and {alpha}-actinin are components of the nephrin multiprotein complex PNAS, July 12, 2005; 102(28): 9814 - 9819. [Abstract] [Full Text] [PDF]

				K. Kimura, J. Kitano, Y. Nakajima, and S. Nakanishi Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction Genes Cells, July 1, 2004; 9(7): 631 - 640. [Abstract] [Full Text] [PDF]

				T. M. Elul, N. E. Kimes, M. Kohwi, and L. F. Reichardt N- and C-Terminal Domains of {beta}-Catenin, Respectively, Are Required to Initiate and Shape Axon Arbors of Retinal Ganglion Cells In Vivo J. Neurosci., July 23, 2003; 23(16): 6567 - 6575. [Abstract] [Full Text] [PDF]

				G. R. Phillips, H. Tanaka, M. Frank, A. Elste, L. Fidler, D. L. Benson, and D. R. Colman {gamma}-Protocadherins Are Targeted to Subsets of Synapses and Intracellular Organelles in Neurons J. Neurosci., June 15, 2003; 23(12): 5096 - 5104. [Abstract] [Full Text] [PDF]

				R. A. Hall and R. J. Lefkowitz Regulation of G Protein-Coupled Receptor Signaling by Scaffold Proteins Circ. Res., October 18, 2002; 91(8): 672 - 680. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help