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The Journal of Neuroscience, April 1, 2002, 22(7):2469-2477
Specificity of Glomerular Targeting by Olfactory Sensory Axons
Helen B. Treloar1, Paul Feinstein2, Peter Mombaerts2, and Charles A. Greer1 1 Department of Neurosurgery and Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06520-8082, and 2 The Rockefeller University, New York, New York 10021
Axons from olfactory sensory neurons (OSNs) expressing a specific odorant receptor (OR) project to specific subsets of glomeruli in the olfactory bulb (for review, see Mombaerts, 1999, 2001). The aim of this study was to examine the trajectories that subsets of axons from OSNs expressing the same OR follow within the olfactory nerve and olfactory nerve layer (ONL) of adult mice. Using confocal microscopy, we generated serial reconstructions of axons from M72-IRES-tauGFP-expressing OSNs as they coursed within the ONL and into glomeruli. GFP-expressing axons were loosely aggregated in the outer ONL; however, as they entered the inner ONL, the majority fasciculated with other GFP-expressing axons before entering the glomerular neuropil. Although the vast majority of axons entered the glomerulus from the directly apposed ONL, some followed tortuous courses through and/or around adjacent glomeruli before terminating in the target glomerulus. Similar observations were made on subpopulations of axons in M71-IRES-tauGFP and P2-IRES-tauGFP mice. Ultrastructural analyses of labeled M72 glomeruli showed no evidence of axodendritic synapses other than those with GFP-labeled axon terminals. These data are consistent with the notion that OSN axons are highly precise in targeting glomeruli and that glomeruli, in turn, are highly homogeneous with regard to the OR expressed by the innervating OSNs. Because some single axons could follow idiosyncratic trajectories to the target glomerulus, it appears that stable homotypic fasciculation is not a prerequisite for correct targeting.
Key words: axon targeting; GFP; electron microscopy; odor receptors; olfaction; synapse
Copyright © 2002 Society for Neuroscience 0270-6474/02/2272469-09$05.00/0
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