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The Journal of Neuroscience, April 1, 2002, 22(7):2513-2521
Cell Type- and Input-Specific Differences in the Number and
Subtypes of Synaptic GABAA Receptors in the Hippocampus
Thomas
Klausberger,
J. David B.
Roberts, and
Peter
Somogyi
Medical Research Council, Anatomical Neuropharmacology Unit,
Department of Pharmacology, Oxford University, Oxford OX1 3TH, United
Kingdom
Networks of parvalbumin (PV)-expressing basket cells are implicated
in synchronizing cortical neurons at various frequencies, through
GABAA receptor-mediated synaptic action. These cells are interconnected by GABAergic synapses and gap junctions, and converge with a different class of cholecystokinin-expressing, PV-negative basket cells onto pyramidal cells. To define the molecular
specializations in the synapses of the two basket cell populations, we
used quantitative electron microscopic immunogold localization of
GABAA receptors. Synapses formed by PV-positive basket
cells on the somata of pyramidal cells had several-fold higher density
of  1 subunit-containing receptors than synapses made by
PV-negative basket cells, most of which were immunonegative. The
density of the  2/3 subunits was similar in the two
populations of synapse, indicating similar overall receptor density.
Synapses interconnecting parvalbumin-expressing basket cells contained
a 3.6 times higher overall density of GABAA receptor
(2/3 subunits) and 3.2 times higher density of
1 subunit labeling compared with synapses formed by
boutons of PV-positive basket cells on pyramidal cells. Thus,
PV-positive basket cells mainly act through 1
subunit-containing GABAA receptors, but the receptor
density depends on the postsynaptic cell type. These observations,
together with previously reported enrichment of the 2
subunit-containing receptors in synapses made by PV-negative basket
cells, indicate that the number and subtypes of GABAA
receptors present in different synapse populations are regulated by
both presynaptic and postsynaptic influences. The high number of
GABAA receptors in synapses on basket cells might
contribute to the precisely timed phasing of basket cell activity.
Key words:
basket cell; pyramidal cell; IPSP; interneuron; inhibition; receptor targeting
Copyright © 2002 Society for Neuroscience 0270-6474/02/2272513-09$05.00/0
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