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The Journal of Neuroscience, January 1, 2003, 23(1):1-6

BRIEF COMMUNICATION
Actin-ATP Hydrolysis Is a Major Energy Drain for Neurons

Barbara W. Bernstein and James R. Bamburg

Department of Biochemistry and Molecular Biology and the Program in Molecular, Cellular, and Integrative Neuroscience, Colorado State University, Fort Collins, Colorado 80523-1870

In cultured chick ciliary neurons, when ATP synthesis is inhibited, ATP depletion is reduced ~50% by slowing actin filament turnover with jasplakinolide or latrunculin A. Jasplakinolide inhibits actin disassembly, and latrunculin A prevents actin assembly by sequestering actin monomers. Cytochalasin D, which allows assembly-disassembly, but only at pointed ends, is less effective in conserving ATP. Ouabain, an Na⁺-K⁺-ATPase inhibitor, and jasplakinolide both prevent ~50% of the ATP loss. When applied together, they completely prevent ATP loss over a period of 20 min, suggesting that filament stabilization reduces ATP consumption by decreasing actin-ATP hydrolysis directly rather than indirectly by modulating the activity of Na⁺-K⁺-ATPase, a major energy consumer.

Key words: ischemia; actin filament treadmilling; intracellular ATP; jasplakinolide; latrunculin; cytoskeleton

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Copyright © 2003 Society for Neuroscience  0270-6474/03/2311-06$05.00/0

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