QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, June 1, 2003, 23(11):4590-4600

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (45) Citing Articles via Google Scholar Google Scholar Articles by Angelastro, J. M. Articles by Greene, L. A. Search for Related Content PubMed PubMed Citation Articles by Angelastro, J. M. Articles by Greene, L. A.

 Previous Article  |  Next Article 

Regulated Expression of ATF5 Is Required for the Progression of Neural Progenitor Cells to Neurons

James M. Angelastro,^1,3 Tatyana N. Ignatova,^4,5 Valery G. Kukekov,^4,5 Dennis A. Steindler,^4,5 George B. Stengren,^1,3 Cathy Mendelsohn,^1,2,3 and Lloyd A. Greene^1,3

¹ Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032, ² Department of Urology, Columbia University College of Physicians and Surgeons, New York, New York 10032, ³ Department of Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, New York 10032, ⁴ Department of Neuroscience, McKnight Brain Institute, Shands Cancer Center, University of Florida, Gainesville, Florida 32610, and ⁵ Department of Neurosurgery, McKnight Brain Institute, Shands Cancer Center, University of Florida, Gainesville, Florida 32610

An important milestone in brain development is the transition of neuroprogenitor cells to postmitotic neurons. We report that the bZIP transcription factor ATF5 plays a major regulatory role in this process. In developing brain ATF5 expression is high within ventricular zones containing neural stem and progenitor cells and is undetectable in postmitotic neurons. In attached clonal neurosphere cultures ATF5 is expressed by neural stem/progenitor cells and is undetectable in tau-positive neurons. In PC12 cell cultures nerve growth factor (NGF) dramatically downregulates endogenous ATF5 protein and transcripts, whereas exogenous ATF5 suppresses NGF-promoted neurite outgrowth. Such inhibition requires the repression of CRE sites. In contrast, loss of function conferred by dominant-negative ATF5 accelerates NGF-promoted neuritogenesis. Exogenous ATF5 also suppresses, and dominant-negative ATF5 and a small-interfering RNA targeted to ATF5 promote, neurogenesis by cultured nestin-positive telencephalic cells. These findings indicate that ATF5 blocks the differentiation of neuroprogenitor cells into neurons and must be downregulated to permit this process to occur.

Key words: ATF5; neural progenitor cells; NGF; neuron; differentiation; ventricular zone

---

Received Nov. 1, 2002; revised Mar. 10, 2003; accepted Mar. 10, 2003.

This article has been cited by other articles:

				G. Li, W. Li, J. M. Angelastro, L. A. Greene, and D. X. Liu Identification of a Novel DNA Binding Site and a Transcriptional Target for Activating Transcription Factor 5 in C6 Glioma and MCF-7 Breast Cancer Cells Mol. Cancer Res., June 1, 2009; 7(6): 933 - 943. [Abstract] [Full Text] [PDF]

				H.-C. Chuang, J.-M. Wang, W.-C. Hsieh, Y. Chang, and I.-J. Su Up-Regulation of Activating Transcription Factor-5 Suppresses SAP Expression to Activate T Cells in Hemophagocytic Syndrome Associated with Epstein-Barr Virus Infection and Immune Disorders Am. J. Pathol., November 1, 2008; 173(5): 1397 - 1405. [Abstract] [Full Text] [PDF]

				J. W-M. Gho, W.-K. Ip, K. Y-Y. Chan, P. T-Y. Law, P. B-S. Lai, and N. Wong Re-Expression of Transcription Factor ATF5 in Hepatocellular Carcinoma Induces G2-M Arrest Cancer Res., August 15, 2008; 68(16): 6743 - 6751. [Abstract] [Full Text] [PDF]

				Y. Wei, J. Jiang, D. Liu, J. Zhou, X. Chen, S. Zhang, H. Zong, X. Yun, and J. Gu Cdc34-mediated Degradation of ATF5 Is Blocked by Cisplatin J. Biol. Chem., July 4, 2008; 283(27): 18773 - 18781. [Abstract] [Full Text] [PDF]

				M. Pascual, M. J. Gomez-Lechon, J. V. Castell, and R. Jover ATF5 Is a Highly Abundant Liver-Enriched Transcription Factor that Cooperates with Constitutive Androstane Receptor in the Transactivation of CYP2B6: Implications in Hepatic Stress Responses Drug Metab. Dispos., June 1, 2008; 36(6): 1063 - 1072. [Abstract] [Full Text] [PDF]

				D. Zhou, L. R. Palam, L. Jiang, J. Narasimhan, K. A. Staschke, and R. C. Wek Phosphorylation of eIF2 Directs ATF5 Translational Control in Response to Diverse Stress Conditions J. Biol. Chem., March 14, 2008; 283(11): 7064 - 7073. [Abstract] [Full Text] [PDF]

				Y. Watatani, K. Ichikawa, N. Nakanishi, M. Fujimoto, H. Takeda, N. Kimura, H. Hirose, S. Takahashi, and Y. Takahashi Stress-induced Translation of ATF5 mRNA Is Regulated by the 5'-Untranslated Region J. Biol. Chem., February 1, 2008; 283(5): 2543 - 2553. [Abstract] [Full Text] [PDF]

				D. R. Lemos, L. Goodspeed, L. Tonelli, M. P. Antoch, S. R. Ojeda, and H. F. Urbanski Evidence for Circadian Regulation of Activating Transcription Factor 5 But Not Tyrosine Hydroxylase by the Chromaffin Cell Clock Endocrinology, December 1, 2007; 148(12): 5811 - 5821. [Abstract] [Full Text] [PDF]

				S. C. Biswas, Y. Shi, A. Sproul, and L. A. Greene Pro-apoptotic Bim Induction in Response to Nerve Growth Factor Deprivation Requires Simultaneous Activation of Three Different Death Signaling Pathways J. Biol. Chem., October 5, 2007; 282(40): 29368 - 29374. [Abstract] [Full Text] [PDF]

				S. C. Biswas, Y. Shi, J.-P. G. Vonsattel, C. L. Leung, C. M. Troy, and L. A. Greene Bim Is Elevated in Alzheimer's Disease Neurons and Is Required for {beta}-Amyloid-Induced Neuronal Apoptosis J. Neurosci., January 24, 2007; 27(4): 893 - 900. [Abstract] [Full Text] [PDF]

				A. Paquin, F. Barnabe-Heider, R. Kageyama, and F. D. Miller CCAAT/Enhancer-Binding Protein Phosphorylation Biases Cortical Precursors to Generate Neurons Rather Than Astrocytes In Vivo J. Neurosci., November 16, 2005; 25(46): 10747 - 10758. [Abstract] [Full Text] [PDF]

				B. J. Stephens, H. Han, V. Gokhale, and D. D. Von Hoff PRL phosphatases as potential molecular targets in cancer Mol. Cancer Ther., November 1, 2005; 4(11): 1653 - 1661. [Abstract] [Full Text] [PDF]

				S. C. Biswas, D. X. Liu, and L. A. Greene Bim Is a Direct Target of a Neuronal E2F-Dependent Apoptotic Pathway J. Neurosci., September 14, 2005; 25(37): 8349 - 8358. [Abstract] [Full Text] [PDF]

				S. S. Vukkadapu, J. M. Belli, K. Ishii, A. G. Jegga, J. J. Hutton, B. J. Aronow, and J. D. Katz Dynamic interaction between T cell-mediated {beta}-cell damage and {beta}-cell repair in the run up to autoimmune diabetes of the NOD mouse Physiol Genomics, April 14, 2005; 21(2): 201 - 211. [Abstract] [Full Text] [PDF]

				J. M. Angelastro, J. L. Mason, T. N. Ignatova, V. G. Kukekov, G. B. Stengren, J. E. Goldman, and L. A. Greene Downregulation of Activating Transcription Factor 5 Is Required for Differentiation of Neural Progenitor Cells into Astrocytes J. Neurosci., April 13, 2005; 25(15): 3889 - 3899. [Abstract] [Full Text] [PDF]

				D. X. Liu, S. C. Biswas, and L. A. Greene B-Myb and C-Myb Play Required Roles in Neuronal Apoptosis Evoked by Nerve Growth Factor Deprivation and DNA Damage J. Neurosci., October 6, 2004; 24(40): 8720 - 8725. [Abstract] [Full Text] [PDF]

				G. Gu, J. M. Wells, D. Dombkowski, F. Preffer, B. Aronow, and D. A. Melton Global expression analysis of gene regulatory pathways during endocrine pancreatic development Development, January 1, 2004; 131(1): 165 - 179. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help