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The Journal of Neuroscience, July 2, 2003, 23(13):5778-5788

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Cellular/Molecular
Barentsz, a New Component of the Staufen-Containing Ribonucleoprotein Particles in Mammalian Cells, Interacts with Staufen in an RNA-Dependent Manner

Paolo Macchi,¹ Sven Kroening,¹ Isabel M. Palacios,² Simona Baldassa,¹ Barbara Grunewald,¹ Concetta Ambrosino,¹ Bernhard Goetze,¹ Andrei Lupas,¹ Daniel St. Johnston,² and Michael Kiebler¹

¹Max-Planck-Institute for Developmental Biology, D-72076 Tübingen, Germany, and ²Wellcome Trust Cancer Research Institute, Department of Genetics, University of Cambridge, Cambridge CB2 1QR, United Kingdom

Staufen1, the mammalian homolog of Drosophila Staufen, assembles into ribonucleoprotein particles (RNPs), which are thought to transport and localize RNA into dendrites of mature hippocampal neurons. We therefore investigated whether additional components of the RNA localization complex besides Staufen are conserved. One candidate is the mammalian homolog of Drosophila Barentsz (Btz), which is essential for the localization of oskar mRNA to the posterior pole of the Drosophila oocyte and is a component of the oskar RNA localization complex along with Staufen. In this study, we report the characterization of mammalian Btz, which behaves like a nucleocytoplasmic shuttling protein. When expressed in the Drosophila egg chamber, mammalian Btz is still able to interact with Drosophila Staufen and reach the posterior pole in the wild-type oocyte, but does not rescue the btz mutant phenotype. Most interestingly, we show by immunoprecipitation assays that Btz interacts with mammalian Staufen in an RNA-dependent manner through a conserved domain, which encompasses the region of homology to the Drosophila Btz protein and contains a novel conserved motif. One candidate for an RNA that mediates this interaction is the dendritically localized brain cytoplasmic 1 transcript. In addition, Btz and Staufen1 colocalize within particles in the cell body and, to a more variable extent, in dendrites of mature hippocampal neurons. Together, our data suggest that the mRNA transport machinery is conserved during evolution, and that mammalian Btz is an additional component of the dendritic RNPs in hippocampal neurons.

Key words: MLN51; Barentsz; Staufen; ribonucleoprotein particles; RNA transport; hippocampal neurons; BC1 RNA

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Received Jan 9, 2003; revised April 18, 2003; accepted April 18, 2003.

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