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The Journal of Neuroscience, July 9, 2003, 23(14):6063-6073

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Light-Evoked Excitatory and Inhibitory Synaptic Inputs to ON and OFF Ganglion Cells in the Mouse Retina

Ji-Jie Pang, Fan Gao, and Samuel M. Wu

Cullen Eye Institute, Baylor College of Medicine, Houston, Texas 77030

Bipolar cell and amacrine cell synaptic inputs to ganglion cells (GCs) in dark-adapted mouse retinas were studied by recording the light-evoked excitatory cation current (I_C) and inhibitory chloride current (I_Cl) under voltage-clamp conditions, and the cell morphology was revealed by Lucifer yellow fluorescence with a confocal microscope. Three types of GCs were identified. (1) ONGCs exhibits no spike activity in darkness, increased spikes in light, sustained inward I_C, sustained outward I_Cl of varying amplitude, and large soma (20–25 µm in diameter) with-cell-like dendritic field 180–350 µm stratifying near 70% of the inner plexiform layer (IPL) depth. (2) Transient OFFGCs (tOFFGCs) exhibit no spike activity in darkness, transient increased spikes at light offset, small sustained outward I_C in light, a large transient inward I_C at light offset, a sustained outward I_Cl, and a morphology similar to the ONGCs except for that their dendrites stratified near 30% of the IPL depth. (3) Sustained OFFGCs exhibit maintained spike activity of 5–10 Hz in darkness, sustained decrease of spikes in light, sustained outward I_C, sustained outward I_Cl, and a morphology similar to the tOFFGCs. By comparing the response thresholds and dynamic ranges of GCs with those of the preganglion cells, our data suggest that the light responses of each type of GCs are mediated by different sets of bipolar cells and amacrine cells. This detailed physiological analysis complements the existing anatomical results and provides new insights on the functional roles of individual synapses in the inner mammalian retina.

Key words: ganglion cells; light responses; rod bipolar cells; M-cone bipolar cells; S-cone bipolar cells; AII amacrine cells; response threshold; dynamic range

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Received Mar. 17, 2003; revised Apr. 29, 2003; accepted May. 1, 2003.

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