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The Journal of Neuroscience, July 23, 2003, 23(16):6627-6637
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Active Transport of the Survival Motor Neuron Protein and the Role of Exon-7 in Cytoplasmic Localization
Honglai L. Zhang,1 Feng Pan,2 Daewha Hong,1,2 Shailesh M. Shenoy,2 Robert H. Singer,2 andGary J. Bassell1 Departments of 1Neuroscience, Rose F. Kennedy Center for Mental Retardation, and 2Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461
Spinal muscular atrophy (SMA) is a neurodegenerative disease caused by deletion and/or mutation of the survival motor neuron protein Gene (SMN1) that results in the expression of a truncated protein lacking the C terminal exon-7. Whereas SMN has been shown to be an important component of diverse ribonucleoprotein (RNP) complexes, its function in neurons is unknown. We hypothesize that the active transport of SMN may be important for neurite outgrowth and that disruption of exon-7 could impair its normal intracellular trafficking. SMN was localized in granules that were associated with cytoskeletal filament systems and distributed throughout neurites and growth cones. Live cell imaging of enhanced green fluorescent protein (EGFP)-SMN granules revealed rapid, bidirectional and cytoskeletal-dependent movements. Exon-7 was necessary for localization of SMN into the cytoplasm but was not sufficient for granule formation and transport. A cytoplasmic targeting signal within exon-7 was identified that could completely redistribute the nuclear protein D-box binding factor 1 into the cytoplasm. Neurons transfected with SMN lacking exon-7 had significantly shorter neurites, a defect that could be rescued by redirecting the exon-7 deletion mutant into neurites by a targeting sequence from growth-associated protein-43. These findings provide the first demonstration of cytoskeletal-based active transport of SMN in neuronal processes and the function of exon-7 in cytoplasmic localization. Such observations provide motivation to investigate possible transport defects or inefficiency of SMN associated RNPs in motor neuron axons in SMA.
Key words: survival motor neuron protein; spinal muscular atrophy; mRNA transport; mRNA localization; active transport; growth cone; neurite outgrowth
Received Apr. 2, 2003; revised May. 16, 2003; accepted May. 29, 2003.
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