QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, September 10, 2003, 23(23):8221-8230

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (24) Citing Articles via Google Scholar Google Scholar Articles by Sepp, K. J. Articles by Auld, V. J. Search for Related Content PubMed PubMed Citation Articles by Sepp, K. J. Articles by Auld, V. J.

 Previous Article  |  Next Article 

Reciprocal Interactions between Neurons and Glia Are Required for Drosophila Peripheral Nervous System Development

Katharine J. Sepp and Vanessa J. Auld

Department of Zoology, University of British Columbia, Vancouver, Canada V6T 1Z4

A major developmental role of peripheral glia is to mediate sensory axon guidance; however, it is not known whether sensory neurons influence peripheral glial development. To determine whether glia and neurons reciprocally interact during embryonic development, we ablated each cell type by overexpressing the apoptosis gene, grim, and observed the effects on peripheral nervous system (PNS) development. When neurons are ablated, glial defects occur as a secondary effect, and vice versa. Therefore glia and neurons are codependent during embryogenesis. To further explore glial-neuronal interactions, we genetically disrupted glial migration or differentiation and observed the secondary effects on sensory neuron development. Glial migration and ensheathment of PNS axons was blocked by overexpression of activated Rho GTPase, a regulator of actin dynamics. Here, sensory axons extended to the CNS without exhibiting gross pathfinding errors. In contrast, disrupting differentiation by expression of dominant-negative Ras GTPase in glia resulted in major sensory axon pathfinding errors, similar to those seen in glial ablations. Glial overexpression of transgenic components of the epidermal growth factor receptor (EGFR) signaling pathway yielded similar sensory neuron defects and also downregulated the expression of the glial marker Neuroglian. Mutant analysis also suggested that the EGFR ligands Spitz and Vein play roles in peripheral glial development. The observations support a model in which glia express genes necessary for sensory neuron development, and these genes are potentially under the control of the EGFR/Ras signaling pathway.

Key words: Grim; EGFR; Spitz; Vein; Pointed; Yan; Neuroglian

---

Received Dec 16, 2002; revised June 9, 2003; accepted June 12, 2003.

This article has been cited by other articles:

				M. Palgi, R. Lindstrom, J. Peranen, T. P. Piepponen, M. Saarma, and T. I. Heino Evidence that DmMANF is an invertebrate neurotrophic factor supporting dopaminergic neurons PNAS, February 17, 2009; 106(7): 2429 - 2434. [Abstract] [Full Text] [PDF]

				P. E. Micevych, V. Chaban, J. Ogi, P. Dewing, J. K. H. Lu, and K. Sinchak Estradiol Stimulates Progesterone Synthesis in Hypothalamic Astrocyte Cultures Endocrinology, February 1, 2007; 148(2): 782 - 789. [Abstract] [Full Text] [PDF]

				B. Aigouy, V. Van de Bor, M. Boeglin, and A. Giangrande Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation Development, October 15, 2004; 131(20): 5127 - 5138. [Abstract] [Full Text] [PDF]

				R. M. Esper and J. A. Loeb Rapid Axoglial Signaling Mediated by Neuregulin and Neurotrophic Factors J. Neurosci., July 7, 2004; 24(27): 6218 - 6227. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help