QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 8, 2003, 23(27):9208-9219

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (25) Citing Articles via Google Scholar Google Scholar Articles by Goodyear, R. J. Articles by Richardson, G. P. Search for Related Content PubMed PubMed Citation Articles by Goodyear, R. J. Articles by Richardson, G. P.

 Previous Article  |  Next Article 

Cellular/Molecular
A Receptor-Like Inositol Lipid Phosphatase Is Required for the Maturation of Developing Cochlear Hair Bundles

R. J. Goodyear,¹ P. K. Legan,¹ M. B. Wright,² W. Marcotti,¹ A. Oganesian,² S. A. Coats,² C. J. Booth,² C. J. Kros,¹ R. A. Seifert,² D. F. Bowen-Pope,² and G. P. Richardson¹

¹School of Biological Sciences, University of Sussex, Falmer, Brighton, BN1 9QG, United Kingdom, and ²Department of Pathology, University of Washington, Seattle, Washington 98195

A screen for protein tyrosine phosphatases (PTPs) expressed in the chick inner ear yielded a high proportion of clones encoding an avian ortholog of protein tyrosine phosphatase receptor Q (Ptprq), a receptor-like PTP. Ptprq was first identified as a transcript upregulated in rat kidney in response to glomerular nephritis and has recently been shown to be active against inositol phospholipids. An antibody to the intracellular domain of Ptprq, anti-Ptprq, stains hair bundles in mice and chicks. In the chick ear, the distribution of Ptprq is almost identical to that of the 275 kDa hair-cell antigen (HCA), a component of hair-bundle shaft connectors recognized by a monoclonal antibody (mAb) that stains inner-ear hair bundles and kidney glomeruli. Furthermore, anti-Ptprq immunoblots a 275 kDa polypeptide immunoprecipitated by the anti-HCA mAb from the avian inner ear, indicating that the HCA and Ptprq are likely to be the same molecule. In two transgenic mouse strains with different mutations in Ptprq, anti-Ptprq immunoreactivity cannot be detected in the ear. Shaft connectors are absent from mutant vestibular hair bundles, but the stereocilia forming the hair bundle are not splayed, indicating that shaft connectors are not necessary to hold the stereocilia together; however, the mice show rapid postnatal deterioration in cochlear hair-bundle structure, associated with smaller than normal transducer currents with otherwise normal adaptation properties, a progressive loss of basal-coil cochlear hair cells, and deafness. These results reveal that Ptprq is required for formation of the shaft connectors of the hair bundle, the normal maturation of cochlear hair bundles, and the long-term survival of high-frequency auditory hair cells.

Key words: hair cell; mechanotransduction; stereocilia; development; inositol phosphatase; podocyte

---

Received April 7, 2003; revised August 14, 2003; accepted August 18, 2003.

This article has been cited by other articles:

				J. M. Stapelbroek, T. A. Peters, D. H. A. van Beurden, J. H. A. J. Curfs, A. Joosten, A. J. Beynon, B. M. van Leeuwen, L. M. van der Velden, L. Bull, R. P. Oude Elferink, et al. ATP8B1 is essential for maintaining normal hearing PNAS, June 16, 2009; 106(24): 9709 - 9714. [Abstract] [Full Text] [PDF]

				N. Michalski, V. Michel, A. Bahloul, G. Lefevre, J. Barral, H. Yagi, S. Chardenoux, D. Weil, P. Martin, J.-P. Hardelin, et al. Molecular Characterization of the Ankle-Link Complex in Cochlear Hair Cells and Its Role in the Hair Bundle Functioning J. Neurosci., June 13, 2007; 27(24): 6478 - 6488. [Abstract] [Full Text] [PDF]

				Z. M. Ahmed, R. Goodyear, S. Riazuddin, A. Lagziel, P. K. Legan, M. Behra, S. M. Burgess, K. S. Lilley, E. R. Wilcox, S. Riazuddin, et al. The tip-link antigen, a protein associated with the transduction complex of sensory hair cells, is protocadherin-15. J. Neurosci., June 28, 2006; 26(26): 7022 - 7034. [Abstract] [Full Text] [PDF]

				J. McGee, R. J. Goodyear, D. R. McMillan, E. A. Stauffer, J. R. Holt, K. G. Locke, D. G. Birch, P. K. Legan, P. C. White, E. J. Walsh, et al. The very large G-protein-coupled receptor VLGR1: a component of the ankle link complex required for the normal development of auditory hair bundles. J. Neurosci., June 14, 2006; 26(24): 6543 - 6553. [Abstract] [Full Text] [PDF]

				M. Senften, M. Schwander, P. Kazmierczak, C. Lillo, J.-B. Shin, T. Hasson, G. S. G. Geleoc, P. G. Gillespie, D. Williams, J. R. Holt, et al. Physical and Functional Interaction between Protocadherin 15 and Myosin VIIa in Mechanosensory Hair Cells J. Neurosci., February 15, 2006; 26(7): 2060 - 2071. [Abstract] [Full Text] [PDF]

				C. Sage, M. Huang, K. Karimi, G. Gutierrez, M. A. Vollrath, D.-S. Zhang, J. Garcia-Anoveros, P. W. Hinds, J. T. Corwin, D. P. Corey, et al. Proliferation of Functional Hair Cells in Vivo in the Absence of the Retinoblastoma Protein Science, February 18, 2005; 307(5712): 1114 - 1118. [Abstract] [Full Text] [PDF]

				N. Daudet and J. Lewis Two contrasting roles for Notch activity in chick inner ear development: specification of prosensory patches and lateral inhibition of hair-cell differentiation Development, February 1, 2005; 132(3): 541 - 551. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help