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The Journal of Neuroscience, February 1, 2003, 23(3):758
Ca2+ Signaling in Mouse Cortical Neurons Studied by Two-Photon Imaging and Photoreleased Inositol Triphosphate
Grace E. Stutzmann, Frank M. LaFerla, and Ian Parker Department of Neurobiology and Behavior, University of California, Irvine, Irvine, California 92697-4550
IP3-mediated Ca2+ release is a crucial neuronal signaling mechanism that has not been extensively characterized in the mammalian cerebral cortex. We used two-photon, video-rate microscopy to image Ca2+ signals evoked by photoreleased IP3 in pyramidal neurons of mouse prefrontal cortex. Ca2+ responses to photoreleased IP3 varied greatly between different neurons; however, within IP3-responsive neurons, the soma invariably showed highest sensitivity, with signals increasing nonlinearly with [IP3]. Responses to paired photorelease displayed inhibition, whereas IP3-evoked Ca2+ liberation was potentiated by Ca2+ entry during action potentials and vice versa. IP3-mediated Ca2+ signals strongly inhibited spike firing through activation of K+ membrane conductance. Metabotropic signaling via the phosphoinositide pathway thus serves as a powerful and sustained modulator of excitability in cortical neurons and displays complex reciprocal interactions between electrical and chemical signals.
Key words: calcium signaling; coincidence detection; electrophysiology; flash photolysis; modulation; prefrontal cortex
Copyright © 2003 Society for Neuroscience 0270-6474/03/233758-08$05.00/0
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