QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 19, 2003, 23(33):10622-10632

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (26) Citing Articles via Google Scholar Google Scholar Articles by Prevot, V. Articles by Ojeda, S. R. Search for Related Content PubMed PubMed Citation Articles by Prevot, V. Articles by Ojeda, S. R. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Activation of erbB-1 Signaling in Tanycytes of the Median Eminence Stimulates Transforming Growth Factor ₁ Release via Prostaglandin E₂ Production and Induces Cell Plasticity

Vincent Prevot, Anda Cornea, Alison Mungenast, Gregory Smiley, and Sergio R. Ojeda

Division of Neuroscience, Oregon National Primate Research Center, Oregon Health and Sciences University, Beaverton, Oregon 97006

The activation of transforming growth factor (TGF)-erbB-1 and neuregulin-erbB-4 signaling pathways in hypothalamic astrocytes has been shown to play a key role in the process by which the neuroendocrine brain controls luteinizing hormone-releasing hormone (LHRH) secretion. Earlier studies suggested that tanycytes, an ependymoglial cell type of the median eminence, regulate LHRH release during the estrous cycle by undergoing plastic changes that alternatively allow or prevent direct access of the LHRH nerve terminals to the portal vasculature. Neither the molecules responsible for these plastic changes nor the underlying controlling mechanisms have been identified. Here we show that cultured tanycytes express erbB-1 and erbB-2, two of the four members of the erbB receptor family, and respond to TGF with receptor phosphorylation, release of prostaglandin E₂ (PGE₂), and a PGE₂-dependent increase in the release of TGF₁, a growth factor previously implicated in the glial control of LHRH secretion. Blockade of either erbB-1 receptor signal transduction or prostaglandin synthesis prevented the stimulatory effect of TGF on both PGE₂ and TGF₁ release. Time-lapse studies revealed that TGF and TGF₁ have dramatically opposite effects on tanycyte plasticity. Whereas TGF promotes tanycytic outgrowth, TGF₁ elicits retraction of tanycytic processes. Blockade of metalloproteinase activity abolished the effect of TGF₁, suggesting that TGF₁ induces tanycytic retraction by facilitating dissolution of the extracellular matrix. Prolonged (>12 hr) exposure of tanycytes to TGF resulted in focal tanycytic retraction, an effect that was abolished by immunoneutralization of TGF₁ action, indicating that the retraction was attributable to TGF-induced TGF₁ formation. These in vitro results identify tanycytes as targets of TGF action and demonstrate that activation of erbB-1-mediated signaling in these cells results in plastic changes that, involving PGE₂ and TGF₁ as downstream effectors, mimic the morphological plasticity displayed by tanycytes during the hours encompassing the preovulatory surge of LHRH.

Key words: neuroendocrine; TGF; TGF; ependymoglial cells; LHRH; cell plasticity; hypothalamus

---

Received July 28, 2003; revised September 23, 2003; accepted September 24, 2003.

This article has been cited by other articles:

				E. J. Tisdale, F. Azizi, and C. R. Artalejo Rab2 Utilizes Glyceraldehyde-3-phosphate Dehydrogenase and Protein Kinase C{iota} to Associate with Microtubules and to Recruit Dynein J. Biol. Chem., February 27, 2009; 284(9): 5876 - 5884. [Abstract] [Full Text] [PDF]

				L. M. Garcia-Segura, B. Lorenz, and L. L DonCarlos The role of glia in the hypothalamus: implications for gonadal steroid feedback and reproductive neuroendocrine output Reproduction, April 1, 2008; 135(4): 419 - 429. [Abstract] [Full Text] [PDF]

				T. Takagi, T. Yamamura, T. Anraku, S. Yasuo, N. Nakao, M. Watanabe, M. Iigo, S. Ebihara, and T. Yoshimura Involvement of Transforming Growth Factor {alpha} in the Photoperiodic Regulation of Reproduction in Birds Endocrinology, June 1, 2007; 148(6): 2788 - 2792. [Abstract] [Full Text] [PDF]

				C. Mastronardi, G. G. Smiley, J. Raber, T. Kusakabe, A. Kawaguchi, V. Matagne, A. Dietzel, S. Heger, A. E. Mungenast, R. Cabrera, et al. Deletion of the Ttf1 Gene in Differentiated Neurons Disrupts Female Reproduction without Impairing Basal Ganglia Function J. Neurosci., December 20, 2006; 26(51): 13167 - 13179. [Abstract] [Full Text] [PDF]

				S. R. Ojeda, A. Lomniczi, C. Mastronardi, S. Heger, C. Roth, A.-S. Parent, V. Matagne, and A. E. Mungenast Minireview: The Neuroendocrine Regulation of Puberty: Is the Time Ripe for a Systems Biology Approach? Endocrinology, March 1, 2006; 147(3): 1166 - 1174. [Abstract] [Full Text] [PDF]

				A. Lomniczi, A. Cornea, M. E. Costa, and S. R. Ojeda Hypothalamic Tumor Necrosis Factor-{alpha} Converting Enzyme Mediates Excitatory Amino Acid-Dependent Neuron-to-Glia Signaling in the Neuroendocrine Brain J. Neurosci., January 4, 2006; 26(1): 51 - 62. [Abstract] [Full Text] [PDF]

				G. A. Dissen, C. Garcia-Rudaz, V. Tapia, L. F. Parada, S.-Y. T. Hsu, and S. R. Ojeda Expression of the Insulin Receptor-Related Receptor Is Induced by the Preovulatory Surge of Luteinizing Hormone in Thecal-Interstitial Cells of the Rat Ovary Endocrinology, January 1, 2006; 147(1): 155 - 165. [Abstract] [Full Text] [PDF]

				V. Prevot, A. Lomniczi, G. Corfas, and S. R. Ojeda erbB-1 and erbB-4 Receptors Act in Concert to Facilitate Female Sexual Development and Mature Reproductive Function Endocrinology, March 1, 2005; 146(3): 1465 - 1472. [Abstract] [Full Text] [PDF]

				S. De Seranno, C. Estrella, A. Loyens, A. Cornea, S. R. Ojeda, J.-C. Beauvillain, and V. Prevot Vascular Endothelial Cells Promote Acute Plasticity in Ependymoglial Cells of the Neuroendocrine Brain J. Neurosci., November 17, 2004; 24(46): 10353 - 10363. [Abstract] [Full Text] [PDF]

				L. Givalois, S. Arancibia, G. Alonso, and L. Tapia-Arancibia Expression of Brain-Derived Neurotrophic Factor and Its Receptors in the Median Eminence Cells with Sensitivity to Stress Endocrinology, October 1, 2004; 145(10): 4737 - 4747. [Abstract] [Full Text] [PDF]

				S. Bouret, S. De Seranno, J.-C. Beauvillain, and V. Prevot Transforming Growth Factor {beta}1 May Directly Influence Gonadotropin-Releasing Hormone Gene Expression in the Rat Hypothalamus Endocrinology, April 1, 2004; 145(4): 1794 - 1801. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help