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The Journal of Neuroscience, November 19, 2003, 23(33):10650-10661

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Cellular/Molecular
Perisynaptic Localization of {delta} Subunit-Containing GABAA Receptors and Their Activation by GABA Spillover in the Mouse Dentate Gyrus

Weizheng Wei,1,3 Nianhui Zhang,2,3 Zechun Peng,2,3 Carolyn R. Houser,2,3,4 and Istvan Mody1,3

Departments of 1Neurology and Physiology and 2Neurobiology, and 3Brain Research Institute, The David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California 90095, and 4Research Service, Department of Veterans Affairs Greater Los Angeles Healthcare System, West Los Angeles, Los Angeles, California 90073

In cerebellar granule cells, {delta} subunit-containing GABAA receptors are found exclusively at extrasynaptic sites, but their subcellular distribution in other brain areas is poorly understood. We examined the anatomical localization and physiological activation of these receptors in adult mouse dentate gyrus granule cells. Immunocytochemistry revealed a high density of {delta} subunits in the molecular layer and a much lower density in the cell body layer. At the ultrastructural level, immunogold-labeled {delta} subunits were found at the edge of symmetric synapses on granule cell dendrites. Functional correlates of this perisynaptic localization were obtained by comparing inhibitory responses in {delta} subunit-deficient ({delta}-/-) and wild-type (wt) mice. In whole-cell recordings at 22°C, the weighted decay time constants ({tau}w) of spontaneous IPSCs (sIPSCs) were significantly longer in wt mice but were similar at 34°C, reflecting the role of temperature-dependent GABA uptake in shaping sIPSC decay. IPSCs evoked by minimal stimulation (eIPSCs) near the somata had similar {tau}w in {delta}-/- and wt mice, but eIPSCs elicited from dendritic sites decayed significantly more slowly in wt mice, consistent with a higher density of {delta} subunit-containing receptors in the molecular layer. The {tau}w of dendritic eIPSCs of wt mice were shortened by ZnCl2 (10 µM), reflecting the high Zn2+ sensitivity of {delta} subunit-containing GABAA receptors, and were prolonged by the GAT-1 GABA transporter inhibitor NO711 (10 µM). Our results demonstrate a perisynaptic localization of {delta} subunit-containing GABAA receptors and indicate that these receptors can be activated by GABA overspill in the molecular layer.

Key words: hippocampus; IPSCs; immunocytochemistry; GABA uptake; postembedding immunogold; zinc

Received Aug 18, 2003; revised September 29, 2003; accepted October 2, 2003.




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