QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, December 3, 2003, 23(35):11026-11035

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (60) Citing Articles via Google Scholar Google Scholar Articles by Kulik, A. Articles by Frotscher, M. Search for Related Content PubMed PubMed Citation Articles by Kulik, A. Articles by Frotscher, M.

 Previous Article  |  Next Article 

Cellular/Molecular
Subcellular Localization of Metabotropic GABA_B Receptor Subunits GABA_B1a/b and GABA_B2 in the Rat Hippocampus

Ákos Kulik,^1,4 Imre Vida,¹ Rafael Luján,² Carola A. Haas,¹ Guillermina López-Bendito,³ Ryuichi Shigemoto,^4,5 and Michael Frotscher¹

¹Department of Anatomy and Cell Biology, University of Freiburg, 79104 Freiburg, Germany, ²Centro Regional de Investigaciones Biomédicas, Facultad de Medicina, Universidad de Castilla-La Mancha, Campus Biosanitario, 02071 Albacete, Spain, ³Department of Human Anatomy and Genetics, University of Oxford, Oxford, OX1 3QX, United Kingdom, ⁴Division of Cerebral Structure, National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, Japan, and ⁵Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan

Metabotropic GABA_B receptors mediate slow inhibitory effects presynaptically and postsynaptically. Using preembedding immunohistochemical methods combined with quantitative analysis of GABA_B receptor subunit immunoreactivity, this study provides a detailed description of the cellular and subcellular localization of GABA_B1a/b and GABA_B2 in the rat hippocampus. At the light microscopic level, an overlapping distribution of GABA_B1a/b and GABA_B2 was revealed in the dendritic layers of the hippocampus. In addition, expression of the GABA_B1a/b subunit was found in somata of CA1 pyramidal cells and of a subset of GABAergic interneurons. At the electron microscopic level, immunoreactivity for both subunits was observed on presynaptic and, more abundantly, on postsynaptic elements. Presynaptically, subunits were mainly detected in the extrasynaptic membrane and occasionally over the presynaptic membrane specialization of putative glutamatergic and, to a lesser extent, GABAergic axon terminals. Postsynaptically, the majority of GABA_B receptor subunits were localized to the extrasynaptic plasma membrane of spines and dendritic shafts of principal cells and shafts of interneuron dendrites. Quantitative analysis revealed enrichment of GABA_B1a/b around putative glutamatergic synapses on spines and an even distribution on dendritic shafts of pyramidal cells contacted by GABAergic boutons. The association of GABA_B receptors with glutamatergic synapses at both presynaptic and postsynaptic sides indicates their intimate involvement in the modulation of glutamatergic neurotransmission. The dominant extrasynaptic localization of GABA_B receptor subunits suggests that their activation is dependent on spillover of GABA requiring simultaneous activity of populations of GABAergic cells as it occurs during population oscillations or epileptic seizures.

Key words: GABA_B1; GABA_B2; G-protein-coupled receptors; immunocytochemistry labeling; electron microscopy; inhibition; spill-over

---

Received Aug 8, 2003; revised October 6, 2003; accepted October 7, 2003.

This article has been cited by other articles:

				O. A. Ramirez, R. L. Vidal, J. A. Tello, K. J. Vargas, S. Kindler, S. Hartel, and A. Couve Dendritic Assembly of Heteromeric {gamma}-Aminobutyric Acid Type B Receptor Subunits in Hippocampal Neurons J. Biol. Chem., May 8, 2009; 284(19): 13077 - 13085. [Abstract] [Full Text] [PDF]

				N. Guetg, R. Seddik, R. Vigot, R. Turecek, M. Gassmann, K. E. Vogt, H. Brauner-Osborne, R. Shigemoto, O. Kretz, M. Frotscher, et al. The GABAB1a Isoform Mediates Heterosynaptic Depression at Hippocampal Mossy Fiber Synapses J. Neurosci., February 4, 2009; 29(5): 1414 - 1423. [Abstract] [Full Text] [PDF]

				H. J. Chung, W.-P. Ge, X. Qian, O. Wiser, Y. N. Jan, and L. Y. Jan G protein-activated inwardly rectifying potassium channels mediate depotentiation of long-term potentiation PNAS, January 13, 2009; 106(2): 635 - 640. [Abstract] [Full Text] [PDF]

				H. J. Chung, X. Qian, M. Ehlers, Y. N. Jan, and L. Y. Jan Neuronal activity regulates phosphorylation-dependent surface delivery of G protein-activated inwardly rectifying potassium channels PNAS, January 13, 2009; 106(2): 629 - 634. [Abstract] [Full Text] [PDF]

				K. J. Vargas, M. Terunuma, J. A. Tello, M. N. Pangalos, S. J. Moss, and A. Couve The Availability of Surface GABAB Receptors Is Independent of {gamma}-Aminobutyric Acid but Controlled by Glutamate in Central Neurons J. Biol. Chem., September 5, 2008; 283(36): 24641 - 24648. [Abstract] [Full Text] [PDF]

				D.-P. Li, Q. Yang, H.-M. Pan, and H.-L. Pan Plasticity of pre- and postsynaptic GABAB receptor function in the paraventricular nucleus in spontaneously hypertensive rats Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H807 - H815. [Abstract] [Full Text] [PDF]

				L. A. Grande, J. D. Loeser, and A. Samii Recurrent Transient Global Amnesia with Intrathecal Baclofen Anesth. Analg., April 1, 2008; 106(4): 1284 - 1287. [Abstract] [Full Text] [PDF]

				K. Kaneda, P. Phongphanphanee, T. Katoh, K. Isa, Y. Yanagawa, K. Obata, and T. Isa Regulation of Burst Activity through Presynaptic and Postsynaptic GABAB Receptors in Mouse Superior Colliculus J. Neurosci., January 23, 2008; 28(4): 816 - 827. [Abstract] [Full Text] [PDF]

				J. Szabadics, G. Tamas, and I. Soltesz Different transmitter transients underlie presynaptic cell type specificity of GABAA,slow and GABAA,fast PNAS, September 11, 2007; 104(37): 14831 - 14836. [Abstract] [Full Text] [PDF]

				U. Kim and L.-y. Chung Dual GABAergic Synaptic Response of Fast Excitation and Slow Inhibition in the Medial Habenula of Rat Epithalamus J Neurophysiol, September 1, 2007; 98(3): 1323 - 1332. [Abstract] [Full Text] [PDF]

				H. Alle and J. R. P. Geiger GABAergic Spill-Over Transmission onto Hippocampal Mossy Fiber Boutons J. Neurosci., January 24, 2007; 27(4): 942 - 950. [Abstract] [Full Text] [PDF]

				R. M. Lober, M. A. Pereira, and N. A. Lambert Rapid Activation of Inwardly Rectifying Potassium Channels by Immobile G-Protein-Coupled Receptors J. Neurosci., November 29, 2006; 26(48): 12602 - 12608. [Abstract] [Full Text] [PDF]

				C. L. Marker, R. Lujan, J. Colon, and K. Wickman Distinct Populations of Spinal Cord Lamina II Interneurons Expressing G-Protein-Gated Potassium Channels. J. Neurosci., November 22, 2006; 26(47): 12251 - 12259. [Abstract] [Full Text] [PDF]

				A. Ajima and S. Tanaka Spatial Patterns of Excitation and Inhibition Evoked by Lateral Connectivity in Layer 2/3 of Rat Barrel Cortex Cereb Cortex, August 1, 2006; 16(8): 1202 - 1211. [Abstract] [Full Text] [PDF]

				P. Ramoino, L. Gallus, F. Beltrame, A. Diaspro, M. Fato, P. Rubini, S. Stigliani, G. Bonanno, and C. Usai Endocytosis of GABAB receptors modulates membrane excitability in the single-celled organism Paramecium J. Cell Sci., May 15, 2006; 119(10): 2056 - 2064. [Abstract] [Full Text] [PDF]

				A. Kulik, I. Vida, Y. Fukazawa, N. Guetg, Y. Kasugai, C. L. Marker, F. Rigato, B. Bettler, K. Wickman, M. Frotscher, et al. Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells. J. Neurosci., April 19, 2006; 26(16): 4289 - 4297. [Abstract] [Full Text] [PDF]

				I. Cohen, G. Huberfeld, and R. Miles Emergence of disinhibition-induced synchrony in the CA3 region of the guinea pig hippocampus in vitro J. Physiol., February 1, 2006; 570(3): 583 - 594. [Abstract] [Full Text] [PDF]

				L. Koyrakh, R. Lujan, J. Colon, C. Karschin, Y. Kurachi, A. Karschin, and K. Wickman Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels J. Neurosci., December 7, 2005; 25(49): 11468 - 11478. [Abstract] [Full Text] [PDF]

				C. J. Price, B. Cauli, E. R. Kovacs, A. Kulik, B. Lambolez, R. Shigemoto, and M. Capogna Neurogliaform Neurons Form a Novel Inhibitory Network in the Hippocampal CA1 Area J. Neurosci., July 20, 2005; 25(29): 6775 - 6786. [Abstract] [Full Text] [PDF]

				K. Kanbara, K. Okamoto, S. Nomura, T. Kaneko, R. Shigemoto, H. Azuma, Y. Katsuoka, and M. Watanabe Cellular Localization of GABA and GABAB Receptor Subunit Proteins During Spermiogenesis in Rat Testis J Androl, July 1, 2005; 26(4): 485 - 493. [Abstract] [Full Text] [PDF]

				X. Chen and D. Johnston Constitutively Active G-Protein-Gated Inwardly Rectifying K+ Channels in Dendrites of Hippocampal CA1 Pyramidal Neurons J. Neurosci., April 13, 2005; 25(15): 3787 - 3792. [Abstract] [Full Text] [PDF]

				N. A. Otmakhova and J. E. Lisman Contribution of Ih and GABAB to Synaptically Induced Afterhyperpolarizations in CA1: A Brake on the NMDA Response J Neurophysiol, October 1, 2004; 92(4): 2027 - 2039. [Abstract] [Full Text] [PDF]

				M. Gassmann, H. Shaban, R. Vigot, G. Sansig, C. Haller, S. Barbieri, Y. Humeau, V. Schuler, M. Muller, B. Kinzel, et al. Redistribution of GABAB(1) Protein and Atypical GABAB Responses in GABAB(2)-Deficient Mice J. Neurosci., July 7, 2004; 24(27): 6086 - 6097. [Abstract] [Full Text] [PDF]

				S. Balasubramanian, J. A. Teissere, D. V. Raju, and R. A. Hall Hetero-oligomerization between GABAA and GABAB Receptors Regulates GABAB Receptor Trafficking J. Biol. Chem., April 30, 2004; 279(18): 18840 - 18850. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help