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The Journal of Neuroscience, December 10, 2003, 23(36):11315-11321

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Behavioral/Systems/Cognitive
Pivotal Role of Nucleotide P2X₂ Receptor Subunit of the ATP-Gated Ion Channel Mediating Ventilatory Responses to Hypoxia

Weifang Rong,^1 * Alexander V. Gourine,^1 * Debra A. Cockayne,² Zhenghua Xiang,¹ Anthony P. D. W. Ford,² K. Michael Spyer,¹ and Geoffrey Burnstock¹

¹Autonomic Neuroscience Institute and Department of Physiology, Royal Free and University College Medical School, London NW3 2PF, United Kingdom, and ²The Neurobiology Unit, Roche Bioscience, Palo Alto, California 94304

In mammals, the ventilatory response to decreased oxygen tension in the arterial blood is initiated by excitation of specialized O₂-sensitive chemoreceptor cells in the carotid body that release neurotransmitters to activate endings of the sinus nerve afferent fibers. We investigated the role of ATP acting via ionotropic P2X receptors in the carotid body function and ventilatory response to hypoxia in mice. Mice deficient in P2X₂ receptor subunit showed a markedly attenuated ventilatory response to hypoxia, whereas the response to hypoxia in P2X₃-deficient mice was comparable with that seen in wild-type controls. P2X₂ and P2X₃ receptor subunit deficiency did not affect the ventilatory responses to hypercapnia. P2X₂ subunit deficiency resulted in a dramatic reduction in the responses of the carotid sinus nerve to hypoxia in the in vitro carotid body-sinus nerve preparation. ATP and its stable analog ,-methyleneATP both evoked rapid excitation of sinus nerve afferents, and the P2 receptor antagonist PPADS (pyridoxal-5'-phosphate-6-azophenyl-2',4'-disulphonic acid) (100 µM) blocked hypoxia-induced increase in sinus nerve discharge. Immunoreactivities for P2X₂ and P2X₃ subunits were both detected on afferent terminals surrounding clusters of glomus cells in the wild-type animals but were absent in mice deficient in P2X₂ and P2X₃ receptor subunits. These observations provide the first definitive evidence that, in the carotid body, ATP is a key transmitter released by chemoreceptor cells to activate endings of the sinus nerve afferent fibers. We conclude that P2X receptors containing the P2X₂ subunit play a pivotal role in carotid body function and in mediating ventilatory responses to hypoxia.

Key words: ATP; carotid body; chemosensitivity; hypercapnia; hypoxia; knock-out; P2X; purines; respiration; ventilation

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Received Aug 11, 2003; revised September 25, 2003; accepted October 6, 2003.

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