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The Journal of Neuroscience, April 1, 2003, 23(7):2608

Electrophysiological Characterization of Voltage-Gated Currents in Defined Taste Cell Types of Mice

Kathryn F. Medler1, 2, Robert F. Margolskee3, and Sue C. Kinnamon1, 2

1 Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado 80523, 2 Rocky Mountain Taste and Smell Center, University of Colorado Health Sciences Center, Denver, Colorado 80262, and 3 Howard Hughes Medical Institute and Department of Physiology and Biophysics, Mount Sinai School of Medicine, New York, New York 10029

Despite extensive immunological characterization of the cells within taste buds, little is known about the functional significance of the different cell types. In this study, we use taste cells isolated from mouse vallate and foliate papillae to characterize voltage-gated currents in the three principal elongate types of taste cells: type I, II, and III. Cell types are identified by using antibodies to external epitopes [antigen H for type I cells, antigen A for type II cells, and neural cell adhesion molecule (NCAM) for type III cells]. In addition, we identify the subset of type II cells that contains alpha -gustducin, a G-protein involved in bitter transduction, by using transgenic mice expressing green fluorescent protein under the control of the gustducin promoter. Our results indicate that antigen H-immunoreactive (-IR) cells and many of the antigen A-IR cells have small voltage-gated inward Na+ and outward K+ currents but no voltage-gated Ca2+ currents. In contrast, a subset of antigen A-IR cells and all NCAM-IR cells have large inward Na+ and outward K+ currents as well as voltage-gated Ca2+ currents. Unexpectedly, all gustducin-expressing cells lacked voltage-gated Ca2+ currents, suggesting that these cells use mechanisms other than classical synapses to communicate signals to the brain.

Key words: taste-cell types; gustducin; voltage-gated currents; patch-clamp electrophysiology; immunocytochemistry; taste transduction

Copyright © 2003 Society for Neuroscience  0270-6474/03/2372608-10$05.00/0


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