QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, January 7, 2004, 24(1):103-111; doi:10.1523/JNEUROSCI.4473-03.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (33) Citing Articles via Google Scholar Google Scholar Articles by Huang, Y. H. Articles by Bergles, D. E. Search for Related Content PubMed PubMed Citation Articles by Huang, Y. H. Articles by Bergles, D. E. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Substance via MeSH

 Previous Article  |  Next Article 

Cellular/Molecular
Climbing Fiber Activation of EAAT4 Transporters and Kainate Receptors in Cerebellar Purkinje Cells

Yanhua H. Huang,¹ Margaret Dykes-Hoberg,² Kohichi Tanaka,³ Jeffrey D. Rothstein,² and Dwight E. Bergles¹

Departments of ¹Neuroscience and ²Neurology, Johns Hopkins University, Baltimore, Maryland 21205, and ³Laboratory of Molecular Neuroscience, School of Biomedical Science and Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan 113-8510

Cerebellar Purkinje cells (PCs) express two glutamate transporters, EAAC1 (EAAT3) and EAAT4; however, their relative contribution to the uptake of glutamate at synapses is not known. We found that glutamate transporter currents recorded at climbing fiber (CF)-PC synapses are absent in mice lacking EAAT4 but unchanged in mice lacking EAAC1, indicating that EAAT4 is preferentially involved in clearing glutamate from CF synapses. However, comparison of CF synaptic currents between wild-type and transporter knock-out mice revealed that ionotropic glutamate receptors are responsible for >40% of the current previously attributed to transporters, indicating that PCs remove <10% of the glutamate released by the CF. The receptors responsible for the nontransporter component accounted for 5% of the CF EPSC, had a slower time course and lower occupancy than AMPA receptors at CF synapses, and exhibited pharmacological properties consistent with kainate receptors. In GluR5 knock-out mice, this current was dramatically reduced, indicating that CF excitation of PCs involves two distinct classes of ionotropic glutamate receptors, AMPA receptors and GluR5-containing kainate receptors.

Key words: cerebellum; EAAT4; EAAC1; Purkinje neuron; GluR5; kainate receptor; EPSC; glutamate transporter

---

Received Oct 1, 2003; revised November 3, 2003; accepted November 3, 2003.

This article has been cited by other articles:

				J. H. Shin, Y. S. Kim, and D. J. Linden Dendritic glutamate release produces autocrine activation of mGluR1 in cerebellar Purkinje cells PNAS, January 15, 2008; 105(2): 746 - 750. [Abstract] [Full Text] [PDF]

				D. Torres-Salazar and C. Fahlke Neuronal Glutamate Transporters Vary in Substrate Transport Rate but Not in Unitary Anion Channel Conductance J. Biol. Chem., November 30, 2007; 282(48): 34719 - 34726. [Abstract] [Full Text] [PDF]

				M. Renzi, M. Farrant, and S. G. Cull-Candy Climbing-fibre activation of NMDA receptors in Purkinje cells of adult mice J. Physiol., November 15, 2007; 585(1): 91 - 101. [Abstract] [Full Text] [PDF]

				C. Piochon, T. Irinopoulou, D. Brusciano, Y. Bailly, J. Mariani, and C. Levenes NMDA Receptor Contribution to the Climbing Fiber Response in the Adult Mouse Purkinje Cell J. Neurosci., October 3, 2007; 27(40): 10797 - 10809. [Abstract] [Full Text] [PDF]

				K. Shimamoto, Y. Otsubo, Y. Shigeri, Y. Yasuda-Kamatani, M. Satoh, S. Kaneko, and T. Nakagawa Characterization of the Tritium-Labeled Analog of L-threo-beta-Benzyloxyaspartate Binding to Glutamate Transporters Mol. Pharmacol., January 1, 2007; 71(1): 294 - 302. [Abstract] [Full Text] [PDF]

				E. Glowatzki, N. Cheng, H. Hiel, E. Yi, K. Tanaka, G. C. R. Ellis-Davies, J. D. Rothstein, and D. E. Bergles The glutamate-aspartate transporter GLAST mediates glutamate uptake at inner hair cell afferent synapses in the mammalian cochlea. J. Neurosci., July 19, 2006; 26(29): 7659 - 7664. [Abstract] [Full Text] [PDF]

				Y. Takayasu, M. Iino, K. Shimamoto, K. Tanaka, and S. Ozawa Glial glutamate transporters maintain one-to-one relationship at the climbing fiber-Purkinje cell synapse by preventing glutamate spillover. J. Neurosci., June 14, 2006; 26(24): 6563 - 6572. [Abstract] [Full Text] [PDF]

				J. I. Wadiche, A. V. Tzingounis, and C. E. Jahr Intrinsic kinetics determine the time course of neuronal synaptic transporter currents PNAS, January 24, 2006; 103(4): 1083 - 1087. [Abstract] [Full Text] [PDF]

				N. Melzer, D. Torres-Salazar, and C. Fahlke A dynamic switch between inhibitory and excitatory currents in a neuronal glutamate transporter PNAS, December 27, 2005; 102(52): 19214 - 19218. [Abstract] [Full Text] [PDF]

				Y. Takayasu, M. Iino, W. Kakegawa, H. Maeno, K. Watase, K. Wada, D. Yanagihara, T. Miyazaki, O. Komine, M. Watanabe, et al. Differential Roles of Glial and Neuronal Glutamate Transporters in Purkinje Cell Synapses J. Neurosci., September 21, 2005; 25(38): 8788 - 8793. [Abstract] [Full Text] [PDF]

				K. Ito, A. Contractor, and G. T. Swanson Attenuated Plasticity of Postsynaptic Kainate Receptors in Hippocampal CA3 Pyramidal Neurons J. Neurosci., July 7, 2004; 24(27): 6228 - 6236. [Abstract] [Full Text] [PDF]

				Y. H. Huang, S. R. Sinha, K. Tanaka, J. D. Rothstein, and D. E. Bergles Astrocyte Glutamate Transporters Regulate Metabotropic Glutamate Receptor-Mediated Excitation of Hippocampal Interneurons J. Neurosci., May 12, 2004; 24(19): 4551 - 4559. [Abstract] [Full Text] [PDF]

				G. Brasnjo and T. S. Otis Isolation of glutamate transport-coupled charge flux and estimation of glutamate uptake at the climbing fiber-Purkinje cell synapse PNAS, April 20, 2004; 101(16): 6273 - 6278. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help