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The Journal of Neuroscience, January 7, 2004, 24(1):207-217; doi:10.1523/JNEUROSCI.1661-03.2004

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Cellular/Molecular
Gephyrin Is Critical for Glycine Receptor Clustering But Not for the Formation of Functional GABAergic Synapses in Hippocampal Neurons

Sabine Lévi, ^* Stephen M. Logan, ^* Kenneth R. Tovar, and Ann Marie Craig

Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110

The role of the scaffolding protein gephyrin at hippocampal inhibitory synapses is not well understood. A previous study (Kneussel et al., 1999) reported a complete loss of synaptic clusters of the major GABA_AR subunits 2 and 2 in hippocampal neurons lacking gephyrin. In contrast, we show here that GABA_AR 2 and 2 subunits do cluster at pyramidal synapses in hippocampal cultures from gephyrin-/- mice, albeit at reduced levels compared with control neurons. Synaptic aggregation of GABA_AR 1 on interneurons was identical between the culture types. Furthermore, we recorded miniature IPSCs (mIPSCs) from gephyrin-/- neurons. Although the mean mIPSC amplitude was reduced (by 23%) compared with control, the frequency of these events was unchanged. Cell surface labeling experiments indicated that gephyrin contributes, in part, to aggregation but not to insertion or stabilization of GABA_AR 2 and 2 in the plasma membrane. Thus, a major gephyrin-independent component of hippocampal inhibitory synapse development must exist. We also report that glycine receptors cluster at GABAergic synapses in a subset of hippocampal interneurons and pyramidal neurons. Unlike GABA_ARs, synaptic clustering of glycine receptors was completely abolished in gephyrin-/- neurons. Finally, artificial extrasynaptic aggregation of GABA_AR was able to redistribute and cocluster gephyrin by a mechanism requiring a neuron-specific modification or intermediary protein. We propose a model of hippocampal inhibitory synapse development in which some GABA_ARs cluster at synapses by a gephyrin-independent mechanism and recruit gephyrin. This clustered gephyrin may then recruit glycine receptors, additional GABA_ARs, and other signal-transducing components.

Key words: gephyrin; GABA receptor; synaptogenesis; glycine receptor; mIPSC; hippocampal culture

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Received Aug 22, 2003; revised October 24, 2003; accepted November 4, 2003.

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