QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, January 7, 2004, 24(1):68-75; doi:10.1523/JNEUROSCI.1666-03.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (41) Citing Articles via Google Scholar Google Scholar Articles by Karatsoreos, I. N. Articles by Silver, R. Search for Related Content PubMed PubMed Citation Articles by Karatsoreos, I. N. Articles by Silver, R. Pubmed/NCBI databases Substance via MeSH

 Previous Article  |  Next Article 

Behavioral/Systems/Cognitive
Phenotype Matters: Identification of Light-Responsive Cells in the Mouse Suprachiasmatic Nucleus

Ilia N. Karatsoreos,¹ Lily Yan,¹ Joseph LeSauter,³ and Rae Silver^1,2,3

Departments of ¹Psychology and ²Anatomy and Cell Biology, Columbia University, New York, New York 10027, and ³Department of Psychology, Barnard College of Columbia University, New York, New York 10027

The suprachiasmatic nucleus (SCN) of the hypothalamus is the neural locus of the circadian clock. To explore the organization of the SCN, two strains of transgenic mice, each bearing a jellyfish green fluorescent protein (GFP) reporter, were used. In one, GFP was driven by the promoter region of the mouse Period1 gene (mPer1) (Per1::GFP mouse), whereas in the other, GFP was inserted in the promoter region of calbindin-D_28K-bacterial artificial chromosome (CalB::GFP mouse). In the latter mouse, GFP-containing SCN cells are immunopositive for gastrin-releasing peptide. In both mouse lines, light-induced Per1 mRNA and Fos are localized to the SCN subregion containing gastrin-releasing peptide. Double-label immunohistochemistry reveals that most gastrin-releasing peptide cells (70%) contain Fos after a brief light pulse. To determine the properties of SCN cells in this light-responsive region, we examined the expression of rhythmic Period genes and proteins. Gastrin-releasing peptide-containing cells do not express detectable rhythms in these key components of the molecular circadian clock. The results support the view that the mammalian SCN is composed of functionally distinct cell groups, of which some are light induced and others are rhythmic with respect to clock gene expression. Furthermore, the findings suggest that gastrin-releasing peptide is a potential mediator of intercellular communication between light-induced and oscillator cells within the SCN.

Key words: circadian rhythms; SCN; GFP reporter; transgenic; GRP; mPer1; clock gene

---

Received Sep 3, 2003; revised October 31, 2003; accepted October 31, 2003.

This article has been cited by other articles:

				N. G. Hatcher, N. Atkins Jr., S. P. Annangudi, A. J. Forbes, N. L. Kelleher, M. U. Gillette, and J. V. Sweedler Mass spectrometry-based discovery of circadian peptides PNAS, August 26, 2008; 105(34): 12527 - 12532. [Abstract] [Full Text] [PDF]

				S. Thammacharoen, T. A. Lutz, N. Geary, and L. Asarian Hindbrain Administration of Estradiol Inhibits Feeding and Activates Estrogen Receptor-{alpha}-Expressing Cells in the Nucleus Tractus Solitarius of Ovariectomized Rats Endocrinology, April 1, 2008; 149(4): 1609 - 1617. [Abstract] [Full Text] [PDF]

				L. Asarian and N. Geary Estradiol Enhances Cholecystokinin-Dependent Lipid-Induced Satiation and Activates Estrogen Receptor-{alpha}-Expressing Cells in the Nucleus Tractus Solitarius of Ovariectomized Rats Endocrinology, December 1, 2007; 148(12): 5656 - 5666. [Abstract] [Full Text] [PDF]

				I. N. Karatsoreos and R. Silver Minireview: The Neuroendocrinology of the Suprachiasmatic Nucleus as a Conductor of Body Time in Mammals Endocrinology, December 1, 2007; 148(12): 5640 - 5647. [Abstract] [Full Text] [PDF]

				I. N. Karatsoreos, A. Wang, J. Sasanian, and R. Silver A Role for Androgens in Regulating Circadian Behavior and the Suprachiasmatic Nucleus Endocrinology, November 1, 2007; 148(11): 5487 - 5495. [Abstract] [Full Text] [PDF]

				K. L. Gamble, G. C. Allen, T. Zhou, and D. G. McMahon Gastrin-Releasing Peptide Mediates Light-Like Resetting of the Suprachiasmatic Nucleus Circadian Pacemaker through cAMP Response Element-Binding Protein and Per1 Activation J. Neurosci., October 31, 2007; 27(44): 12078 - 12087. [Abstract] [Full Text] [PDF]

				K. Y. Wong, D. M. Graham, and D. M. Berson The Retina-Attached SCN Slice Preparation: An In Vitro Mammalian Circadian Visual System J Biol Rhythms, October 1, 2007; 22(5): 400 - 410. [Abstract] [PDF]

				P. Indic, W. J. Schwartz, E. D. Herzog, N. C. Foley, and M. C. Antle Modeling the Behavior of Coupled Cellular Circadian Oscillators in the Suprachiasmatic Nucleus J Biol Rhythms, June 1, 2007; 22(3): 211 - 219. [Abstract] [PDF]

				L. P. Morin SCN Organization Reconsidered J Biol Rhythms, February 1, 2007; 22(1): 3 - 13. [Abstract] [PDF]

				M. C. Antle, N. C. Foley, D. K. Foley, and R. Silver Gates and Oscillators II: Zeitgebers and the Network Model of the Brain Clock J Biol Rhythms, February 1, 2007; 22(1): 14 - 25. [Abstract] [PDF]

				L. Yan, I. Karatsoreos, J. LeSauter, D. K. Welsh, S. Kay, D. Foley, and R. Silver Exploring Spatiotemporal Organization of SCN Circuits Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 527 - 541. [Abstract] [PDF]

				R. D. Romeo, D. Staub, A. M. Jasnow, I. N. Karatsoreos, J. E. Thornton, and B. S. McEwen Dihydrotestosterone Increases Hippocampal N-Methyl-D-Aspartate Binding But Does Not Affect Choline Acetyltransferase Cell Number in the Forebrain or Choline Transporter Levels in the CA1 Region of Adult Male Rats Endocrinology, April 1, 2005; 146(4): 2091 - 2097. [Abstract] [Full Text] [PDF]

				M. C. Antle, L. J. Kriegsfeld, and R. Silver Signaling within the Master Clock of the Brain: Localized Activation of Mitogen-Activated Protein Kinase by Gastrin-Releasing Peptide J. Neurosci., March 9, 2005; 25(10): 2447 - 2454. [Abstract] [Full Text] [PDF]

				F. Geier, S. Becker-Weimann, A. Kramer, and H. Herzel Entrainment in a Model of the Mammalian Circadian Oscillator J Biol Rhythms, February 1, 2005; 20(1): 83 - 93. [Abstract] [PDF]

				M. H. Hastings and E. D. Herzog Clock Genes, Oscillators, and Cellular Networks in the Suprachiasmatic Nuclei J Biol Rhythms, October 1, 2004; 19(5): 400 - 413. [Abstract] [PDF]

				J. Itri, S. Michel, J. A. Waschek, and C. S. Colwell Circadian Rhythm in Inhibitory Synaptic Transmission in the Mouse Suprachiasmatic Nucleus J Neurophysiol, July 1, 2004; 92(1): 311 - 319. [Abstract] [Full Text] [PDF]

				L. J. Kriegsfeld, J. LeSauter, and R. Silver Targeted Microlesions Reveal Novel Organization of the Hamster Suprachiasmatic Nucleus J. Neurosci., March 10, 2004; 24(10): 2449 - 2457. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help