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The Journal of Neuroscience, March 24, 2004, 24(12):2942-2952; doi:10.1523/JNEUROSCI.0092-04.2004

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Cellular/Molecular
Binding Sites of -Secretase Inhibitors in Rodent Brain: Distribution, Postnatal Development, and Effect of Deafferentation

Xiao-Xin Yan,¹ Tong Li,² Cynthia M. Rominger,¹ Shimoga R. Prakash,¹ Philip C. Wong,² Richard E. Olson,¹ Robert Zaczek,¹ and Yu-Wen Li¹

¹Bristol-Myers Squibb Company, Pharmaceutical Research Institute, Neuroscience Drug Discovery, Wallingford, Connecticut 06492, and ²Department of Pathology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

-Secretase is a multimeric complex consisted of presenilins (PSs) and three other proteins. PSs appear to be key contributors for the enzymatic center, the potential target of a number of recently developed -secretase inhibitors. Using radiolabeled and unlabeled inhibitors as ligands, this study was aimed to determine the in situ distribution of -secretase in the brain. Characterization using PS-1 knock-out mouse embryos revealed 50 and 80% reductions of -secretase inhibitor binding density in the heterozygous (PS-1^+/–) and homozygous (PS-1–/–) embryos, respectively, relative to the wild type (PS-1^+/+). The pharmacological profile from competition binding assays suggests that the ligands may target at the N- and C-terminal fragments of PS essential for -secretase activity. In the adult rat brain, the binding sites existed mostly in the forebrain, the cerebellum, and discrete brainstem areas and were particularly abundant in areas rich in neuronal terminals, e.g., olfactory glomeruli, CA3–hilus area, cerebellar molecular layer, and pars reticulata of the substantia nigra. In the developing rat brain, diffuse and elevated expression of binding sites occurred at the early postnatal stage relative to the adult. The possible association of binding sites with neuronal terminals in the adult brain was further investigated after olfactory deafferentation. A significant decrease with subsequent recovery of binding sites was noted in the olfactory glomeruli after chemical damage of the olfactory epithelium. The findings in this study support a physiological role of PS or -secretase complex in neuronal and synaptic development and plasticity.

Key words: presenilins; -secretase inhibitor; -amyloid precursor protein; Alzheimer; neurogenesis; neuroplasticity; autoradiography

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Received Sep 3, 2003; revised January 30, 2004; accepted January 30, 2004.

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