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The Journal of Neuroscience, March 31, 2004, 24(13):3186-3198; doi:10.1523/JNEUROSCI.5146-03.2004

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Cellular/Molecular
Connexin32-Containing Gap Junctions in Schwann Cells at the Internodal Zone of Partial Myelin Compaction and in Schmidt–Lanterman Incisures

Carola Meier,^1,2 Rolf Dermietzel,¹ Kimberly G. V. Davidson,² Thomas Yasumura,² and John E. Rash^2,3

¹Department of Neuroanatomy and Molecular Brain Research, Ruhr-University Bochum, 44801 Bochum, Germany, and ²Department of Biomedical Sciences and ³Program in Molecular, Cellular, and Integrative Neurosciences, Colorado State University, Fort Collins, Colorado 80523

In vertebrate peripheral nerves, the insulating myelin sheath is formed by Schwann cells, which generate flattened membrane processes that spiral around axons and form compact myelin by extrusion of cytoplasm and adhesion of apposed intracellular and extracellular membrane surfaces. Cytoplasm remains within the innermost and outermost tongues, in the paranodal loops bordering nodes of Ranvier and in Schmidt–Lanterman incisures. By immunocytochemistry, connexin32 (Cx32) protein has been demonstrated at paranodal loops and Schmidt–Lanterman incisures, and it is widely assumed that gap junctions are present in these locations, thereby providing a direct radial route for transport of ions and metabolites between cytoplasmic myelin layers. This study used freeze-fracture replica immunogold labeling to detect Cx32 in ultrastructurally defined gap junctions in Schmidt–Lanterman incisures, as well as in a novel location, between the outer two layers of internodal myelin, approximately every micrometer along the entire length of myelin, at the zone between compact myelin and noncompact myelin. Thus, these gap junctions link the partially compacted second layer of myelin to the noncompact outer tongue. Although these gap junctions are unusually small (average, 11 connexon channels), their relative abundance and regular distribution along the zone that is structurally intermediate between compact and noncompact myelin demonstrates the existence of multiple sites for unidirectional or bidirectional transport of water, ions, and small molecules between these two distinct cytoplasmic compartments, possibly to regulate or facilitate myelin compaction or to maintain the transition zone between noncompact and compact myelin.

Key words: Charcot-Marie-Tooth disease; connexon; freeze fracture; immunogold labeling; sciatic nerve; tight junction

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Received Nov 21, 2003; revised February 12, 2004; accepted February 12, 2004.

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