QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, March 31, 2004, 24(13):3421-3435; doi:10.1523/JNEUROSCI.0062-04.2004

This Article Full Text Full Text (PDF) Erratum An erratum has been published Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (21) Citing Articles via Google Scholar Google Scholar Articles by Clark, M. C. Articles by Baro, D. J. Search for Related Content PubMed PubMed Citation Articles by Clark, M. C. Articles by Baro, D. J.

 Previous Article  |  Next Article 

Cellular/Molecular
Arthropod 5-HT₂ Receptors: A Neurohormonal Receptor in Decapod Crustaceans That Displays Agonist Independent Activity Resulting from an Evolutionary Alteration to the DRY Motif

Merry C. Clark,¹ Timothy E. Dever,¹ John J. Dever,¹ Ping Xu,¹ Vincent Rehder,¹ Maria A. Sosa,^2,4 and Deborah J. Baro^1,2,3

¹Department of Biology, Georgia State University, Atlanta, Georgia 30303, and ²Institute of Neurobiology and Departments of ³Biochemistry and ⁴Anatomy, University of Puerto Rico Medical Sciences Campus, San Juan, Puerto Rico 00901

The stomatogastric nervous system (STNS) is a premiere model for studying modulation of motor pattern generation. Whereas the cellular and network responses to monoamines have been particularly well characterized electrophysiologically, the transduction mechanisms that link the different monoaminergic signals to specific intracellular responses are presently unknown in this system. To begin to elucidate monoaminergic signal transduction in pyloric neurons, we used a bioinformatics approach to predict the existence of 18 monoamine receptors in arthropods, 9 of which have been previously cloned in Drosophila and other insects. We then went on to use the two existing insect databases to clone and characterize the 10th putative arthropod receptor from the spiny lobster, Panulirus interruptus. This receptor is most homologous to the 5-HT₂ subtype and shows a dose-dependent response to 5-HT but not to any of the other monoamines present in the STNS. Through a series of pharmacological experiments, we demonstrate that this newly described receptor, 5-HT_2Pan, couples with the traditional G_q pathway when expressed in HEK293 cells, but not to G_s or G_i/o. Moreover, it is constitutively active, because the highly conserved DRY motif in transmembrane region 3 has evolved into DRF. Site-directed mutagenesis that reverts the motif back to DRY abolishes this agonist-independent activity. We further demonstrate that this receptor most likely participates in the modulation of stomatogastric motor output, because it is found in neurites in the synaptic neuropil of the stomatogastric ganglion as well as in the axon terminals at identified pyloric neuromuscular junctions.

Key words: STG; constitutive; Panulirus interruptus; G_q; neuromodulator; monoamine; immunocytochemistry; PLC; signal transduction; PKC; IP₃; cAMP; serotonin; stomatogastric; motor pattern; CPG; GPCR

---

Received Jan 7, 2004; revised February 16, 2004; accepted February 18, 2004.

This article has been cited by other articles:

				M. Zhang, X.-W. Fei, Y.-L. He, G. Yang, and Y.-A. Mei Bradykinin inhibits the transient outward K+ current in mouse Schwann cells via the cAMP/PKA pathway Am J Physiol Cell Physiol, June 1, 2009; 296(6): C1364 - C1372. [Abstract] [Full Text] [PDF]

				H. Dircksen Insect ion transport peptides are derived from alternatively spliced genes and differentially expressed in the central and peripheral nervous system J. Exp. Biol., February 1, 2009; 212(3): 401 - 412. [Abstract] [Full Text] [PDF]

				N. Spitzer, G. Cymbalyuk, H. Zhang, D. H. Edwards, and D. J. Baro Serotonin Transduction Cascades Mediate Variable Changes in Pyloric Network Cycle Frequency in Response to the Same Modulatory Challenge J Neurophysiol, June 1, 2008; 99(6): 2844 - 2863. [Abstract] [Full Text] [PDF]

				N. Spitzer, D. H. Edwards, and D. J. Baro Conservation of structure, signaling and pharmacology between two serotonin receptor subtypes from decapod crustaceans, Panulirus interruptus and Procambarus clarkii J. Exp. Biol., January 1, 2008; 211(1): 92 - 105. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help