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The Journal of Neuroscience, January 14, 2004, 24(2):576-587; doi:10.1523/JNEUROSCI.4529-03.2004
Development/Plasticity/Repair
Control of Cortical Neuron Migration and Layering: Cell and Non Cell-Autonomous Effects of p35
Vicki Hammond,1 Li-Huei Tsai,2 andSeong-Seng Tan1 1Howard Florey Institute, The University of Melbourne, Victoria 3010, Australia, and 2Department of Pathology and Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts 02115
The migration, arrest, and ultimately positioning of cortical neurons require signaling activity from Reelin as well as from cyclin-dependent kinase 5 (Cdk5). Although both molecules control neuronal positioning, they achieve their effects by quite separate molecular pathways. Cdk5 is a serine-threonine kinase, the activity of which is dependent on its activating subunits p35 and p39. Mice deficient in Cdk5, p35, or both p35 and p39 display the hallmarks of disturbed cortical development, including cortical layer inversion, neuronal disorientation, and abnormal fiber infiltration. To distinguish between the cell- and non cell-autonomous functions of p35, we constructed p35+/+
p35-/- chimeras using the lacZ gene as an independent marker for p35+/+ cells. In this shared developmental space, wild-type and mutant neurons behaved cell-autonomously with respect to layering. Wild-type cells formed a properly layered supercortex that is mirrored by an inverted mutant cortex lying underneath. However, this genotype-specific behavior was confined to the pyramidal population, and interneurons belonging to either genotype were indiscriminately distributed. However, there was also non cell-autonomous rescue of mutant neurons, and this rescue was specific only to early-born pyramidal neurons belonging to layer V. Rescued neurons reached the correct layer address and possessed appropriate neuronal morphology, orientation, and projections. Later-born neurons belonging to layers II and III were not rescued. These results demonstrate that p35 signaling can have both cell- and non cell-autonomous consequences, and their effects are not uniformly shared by cortical neurons born at different times or born at different places (projection neurons vs interneurons).
Key words: p35; Cdk5; development; cortex; migration; layering; GABA
Received July 8, 2003; revised November 14, 2003; accepted November 21, 2003.
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