 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
The Journal of Neuroscience, June 23, 2004, 24(25):5810-5815; doi:10.1523/JNEUROSCI.1109-04.2004
Previous Article | Next Article
Neurobiology of Disease
Stroke Transiently Increases Subventricular Zone Cell Division from Asymmetric to Symmetric and Increases Neuronal Differentiation in the Adult Rat
Ruilan Zhang,1 Zhenggang Zhang,1 Chunling Zhang,1 Li Zhang,1 Adam Robin,1 Ying Wang,1 Mei Lu,2 andMichael Chopp1,3 Departments of 1Neurology and 2Biostatistics and Research Epidemiology, Henry Ford Health Sciences Center, Detroit, Michigan 48202, and 3Department of Physics, Oakland University, Rochester, Michigan 48309
The orientation of mitotic cleavage regulates neurogenesis during neural development. We examined the orientation of mitotic cleavage of dividing progenitor cells in the subventricular zone (SVZ) of adult rats subjected to stroke. In nonstroke rats, 55% of dividing cells were oriented horizontally, whereas 40% were oriented vertically. Horizontal and vertical cleavage orientations produce asymmetric and symmetric divisions, respectively. Four days after stroke, the number of dividing cells increased twofold, whereas the proportion of symmetric dividing cells significantly (p < 0.01) increased from 40% before stroke to 60%. Fourteen days after stroke, the percentage of symmetric dividing cells was 47%. Stroke-increased numbers of dividing cells in M-phase were confirmed by immuostaining. In nonstroke rats, 37 and 33% of symmetric and asymmetric dividing cells, respectively, exhibited a neuronal marker (TuJ1). Four days after stroke, rats exhibited a significant (p < 0.05) augmentation of the frequency (47%) of neuronal distribution showing TuJ1 immunoreactivity in cells with symmetric division but not cells with asymmetric division (33%). Numb immunoreactivity was detected in SVZ cells of nonstroke rats. Stroke did not change Numb distribution. Our data suggest that neurons are produced by both asymmetric and symmetric cell divisions in the adult SVZ, and the transient increases in symmetric division and neuronal differentiation may result in stroke-induced neurogenesis.
Key words: focal cerebral ischemia; cell division; neural progenitor cells; subventricular zone; mitosis; rats
Received Dec 22, 2003; revised May 12, 2004; accepted May 12, 2004.
This article has been cited by other articles:
Y. Liu, R. Lacson, J. Cassaday, D. A. Ross, A. Kreamer, E. Hudak, R. Peltier, D. Mclaren, I. Munoz-Sanjuan, F. Santini, et al.
Identification of Small-Molecule Modulators of Mouse SVZ Progenitor Cell Proliferation and Differentiation Through High-Throughput Screening
J Biomol Screen, April 1, 2009; 14(4): 319 - 329.
[Abstract] [PDF]
L. Kerosuo, K. Piltti, H. Fox, A. Angers-Loustau, V. Hayry, M. Eilers, H. Sariola, and K. Wartiovaara
Myc increases self-renewal in neural progenitor cells through Miz-1
J. Cell Sci., December 1, 2008; 121(23): 3941 - 3950.
[Abstract] [Full Text] [PDF]
S. Pluchino, L. Muzio, J. Imitola, M. Deleidi, C. Alfaro-Cervello, G. Salani, C. Porcheri, E. Brambilla, F. Cavasinni, A. Bergamaschi, et al.
Persistent inflammation alters the function of the endogenous brain stem cell compartment
Brain, October 1, 2008; 131(10): 2564 - 2578.
[Abstract] [Full Text] [PDF]
J. J. Ohab and S. T. Carmichael
Poststroke Neurogenesis: Emerging Principles of Migration and Localization of Immature Neurons
Neuroscientist, August 1, 2008; 14(4): 369 - 380.
[Abstract] [PDF]
J. Kaslin, J. Ganz, and M. Brand
Proliferation, neurogenesis and regeneration in the non-mammalian vertebrate brain
Phil Trans R Soc B, January 12, 2008; 363(1489): 101 - 122.
[Abstract] [Full Text] [PDF]
P. Thored, J. Wood, A. Arvidsson, J. Cammenga, Z. Kokaia, and O. Lindvall
Long-Term Neuroblast Migration Along Blood Vessels in an Area With Transient Angiogenesis and Increased Vascularization After Stroke
Stroke, November 1, 2007; 38(11): 3032 - 3039.
[Abstract] [Full Text] [PDF]
R. Lan Zhang, Y. LeTourneau, S. R. Gregg, Y. Wang, Y. Toh, A. M. Robin, Z. Gang Zhang, and M. Chopp
Neuroblast Division during Migration toward the Ischemic Striatum: A Study of Dynamic Migratory and Proliferative Characteristics of Neuroblasts from the Subventricular Zone
J. Neurosci., March 21, 2007; 27(12): 3157 - 3162.
[Abstract] [Full Text] [PDF]
J. J. Ohab, S. Fleming, A. Blesch, and S. T. Carmichael
A Neurovascular Niche for Neurogenesis after Stroke
J. Neurosci., December 13, 2006; 26(50): 13007 - 13016.
[Abstract] [Full Text] [PDF]
R. J. Felling, M. J. Snyder, M. J. Romanko, R. P. Rothstein, A. N. Ziegler, Z. Yang, M. I. Givogri, E. R. Bongarzone, and S. W. Levison
Neural stem/progenitor cells participate in the regenerative response to perinatal hypoxia/ischemia.
J. Neurosci., April 19, 2006; 26(16): 4359 - 4369.
[Abstract] [Full Text] [PDF]
S.-R. Lee, H.-Y. Kim, J. Rogowska, B.-Q. Zhao, P. Bhide, J. M. Parent, and E. H. Lo
Involvement of matrix metalloproteinase in neuroblast cell migration from the subventricular zone after stroke.
J. Neurosci., March 29, 2006; 26(13): 3491 - 3495.
[Abstract] [Full Text] [PDF]
S. K. Mishra, N. Braun, V. Shukla, M. Fullgrabe, C. Schomerus, H.-W. Korf, C. Gachet, Y. Ikehara, J. Sevigny, S. C. Robson, et al.
Extracellular nucleotide signaling in adult neural stem cells: synergism with growth factor-mediated cellular proliferation
Development, February 15, 2006; 133(4): 675 - 684.
[Abstract] [Full Text] [PDF]
R. L. Zhang, Z. G. Zhang, and M. Chopp
Neurogenesis in the Adult Ischemic Brain: Generation, Migration, Survival, and Restorative Therapy
Neuroscientist, October 1, 2005; 11(5): 408 - 416.
[Abstract] [PDF]
|
|
|
|
 |
|