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The Journal of Neuroscience, July 21, 2004, 24(29):6507-6514; doi:10.1523/JNEUROSCI.3727-03.2004

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Behavioral/Systems/Cognitive
Stereotyped Odor-Evoked Activity in the Mushroom Body of Drosophila Revealed by Green Fluorescent Protein-Based Ca²⁺ Imaging

Yalin Wang,¹ Hui-Fu Guo,¹ Thomas A. Pologruto,^1,2,3 Frances Hannan,¹ Inessa Hakker,¹ Karel Svoboda,^1,2 and Yi Zhong¹

¹Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, and ²Howard Hughes Medical Institute and ³Graduate Program in Biophysics, Harvard University, Boston, Massachusetts 02138

To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca²⁺ sensor, G-CaMP, in the Drosophila mushroom body (MB). Using two-photon microscopy, we imaged odor-evoked G-CaMP fluorescence transients in MB neurons [Kenyon cells (KCs)] with single-cell resolution. Odors produced large fluorescence transients in a subset of KC somata and in restricted regions of the calyx, the neuropil of the MB. In different KCs, odor-evoked fluorescence transients showed diverse changes with odor concentration: in some KCs, fluorescence transients were evoked by an odor at concentrations spanning several orders of magnitude, whereas in others only at a narrow concentration range. Different odors produced fluorescence transients in different subsets of KCs. The spatial distributions of KCs showing fluorescence transients evoked by a given odor were similar across individuals. For some odors, individual KCs with fluorescence transients evoked by a particular odor could be found in similar locations in different flies with spatial precisions on the order of the size of KC somata. These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB.

Key words: odor; stereotype; mushroom body; calcium imaging; G-CaMP; two photon; Kenyon cell

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Received Aug 10, 2003; revised June 1, 2004; accepted June 3, 2004.

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