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The Journal of Neuroscience, September 29, 2004, 24(39):8480-8484; doi:10.1523/JNEUROSCI.1598-04.2004

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Role of T-Type Calcium Current in Identified D-Hair Mechanoreceptor Neurons Studied In Vitro

Anne-Sophie Dubreuil,¹ Hassan Boukhaddaoui,¹ Gilles Desmadryl,¹ Carlos Martinez-Salgado,² Rabih Moshourab,² Gary R. Lewin,² Patrick Carroll,¹ Jean Valmier,¹ and Frédérique Scamps¹

¹Institut National de la Santé et de la Recherche Médicale, Unité 583, Hopital St. Éloi, 34091 Montpellier, France, and ²Growth Factor and Regeneration Group, Max Delbruck Institute for Molecular Medicine, D-13122 Berlin-Buch, Germany

Different subsets of dorsal root ganglion (DRG) mechanoreceptors transduce low- and high-intensity mechanical stimuli. It was shown recently that, in vivo, neurotrophin-4 (NT-4)-dependent D-hair mechanoreceptors specifically express a voltage-activated T-type calcium channel (Ca_v3.2) that may be required for their mechanoreceptive function. Here we show that D-hair mechanoreceptors can be identified in vitro by a rosette-like morphology in the presence of NT-4 and that these rosette neurons are almost all absent in DRG cultures taken from NT-4 knock-out mice. In vitro identification of the D-hair mechanoreceptor allowed us to explore the electrophysiological properties of these cells. We demonstrate that the T-type Ca_v3.2 channel induced slow membrane depolarization that contributes to lower the voltage threshold for action potential generation and controls spike latency after stimulation of D-hair mechanoreceptors. Indeed, the properties of the T-type amplifier are particularly well suited to explain the high sensitivity of D-hair mechanoreceptors to slowly moving stimuli.

Key words: T-type calcium channel; Ca_v3.2; neurotrophin; mechanotransduction; D-hair neuron; dorsal root ganglion

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Received April 27, 2004; revised August 16, 2004; accepted August 16, 2004.

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