 |
The Journal of Neuroscience, January 28, 2004, 24(4):836-842; doi:10.1523/JNEUROSCI.4221-03.2004
Previous Article | Next Article 
Cellular/Molecular
Morphological and Physiological Features of a Set of Spinal Substantia Gelatinosa Neurons Defined by Green Fluorescent Protein Expression
Adam W. Hantman,1
Anthony N. van den Pol,2 and
Edward R. Perl1
1Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, and 2Department of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut 06520
The spinal substantia gelatinosa (SG) is known to be involved in the manipulation of nociceptive and thermal primary afferent input; however, the interrelationships of its neuronal components are poorly understood. As a step toward expanding understanding, we took a relatively unique approach by concentrating on a set of SG neurons selectively labeled by green fluorescent protein (GFP) in a transgenic mouse. These GFP-expressing SG neurons prove to have homogenous morphological and electrophysiological properties, are systematically spaced in the SG, contain GABA, receive C-fiber primary afferent input, and upregulate c-Fos protein in response to noxious stimuli. Together, the properties established for these GFP-labeled neurons are consistent with a modular SG organization in which afferent activity related to nociception or other C-fiber signaling are subject to integration/modulation by repeating, similar circuits of neurons.
Key words: C-fiber; dorsal horn; GABA; nociception; spinal; substantia gelatinosa; pain
Received Sep 15, 2003;
revised November 7, 2003;
accepted November 7, 2003.
This article has been cited by other articles:

|
 |

|
 |
 
H. Wang and M. J. Zylka
Mrgprd-Expressing Polymodal Nociceptive Neurons Innervate Most Known Classes of Substantia Gelatinosa Neurons
J. Neurosci.,
October 21, 2009;
29(42):
13202 - 13209.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
C. A. Daniele and A. B. MacDermott
Low-Threshold Primary Afferent Drive onto GABAergic Interneurons in the Superficial Dorsal Horn of the Mouse
J. Neurosci.,
January 21, 2009;
29(3):
686 - 695.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. J. Maxwell, M. D. Belle, O. Cheunsuang, A. Stewart, and R. Morris
Morphology of inhibitory and excitatory interneurons in superficial laminae of the rat dorsal horn
J. Physiol.,
October 15, 2007;
584(2):
521 - 533.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
V. B. Lu, K. Ballanyi, W. F. Colmers, and P. A. Smith
Neuron type-specific effects of brain-derived neurotrophic factor in rat superficial dorsal horn and their relevance to 'central sensitization'
J. Physiol.,
October 15, 2007;
584(2):
543 - 563.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. A. Graham, A. M. Brichta, and R. J. Callister
Moving From an Averaged to Specific View of Spinal Cord Pain Processing Circuits
J Neurophysiol,
September 1, 2007;
98(3):
1057 - 1063.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Lu and E. R. Perl
Selective action of noradrenaline and serotonin on neurones of the spinal superficial dorsal horn in the rat
J. Physiol.,
July 1, 2007;
582(1):
127 - 136.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Yasaka, G. Kato, H. Furue, M. H. Rashid, M. Sonohata, A. Tamae, Y. Murata, S. Masuko, and M. Yoshimura
Cell-type-specific excitatory and inhibitory circuits involving primary afferents in the substantia gelatinosa of the rat spinal dorsal horn in vitro
J. Physiol.,
June 1, 2007;
581(2):
603 - 618.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. F. A. Santos, S. Rebelo, V. A. Derkach, and B. V. Safronov
Excitatory interneurons dominate sensory processing in the spinal substantia gelatinosa of rat
J. Physiol.,
May 15, 2007;
581(1):
241 - 254.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Kato, Y. Kawasaki, R.-R. Ji, and A. M. Strassman
Differential wiring of local excitatory and inhibitory synaptic inputs to islet cells in rat spinal lamina II demonstrated by laser scanning photostimulation
J. Physiol.,
May 1, 2007;
580(3):
815 - 833.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Schoffnegger, B. Heinke, C. Sommer, and J. Sandkuhler
Physiological properties of spinal lamina II GABAergic neurons in mice following peripheral nerve injury
J. Physiol.,
December 15, 2006;
577(3):
869 - 878.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Balasubramanyan, P. L. Stemkowski, M. J. Stebbing, and P. A. Smith
Sciatic Chronic Constriction Injury Produces Cell-Type-Specific Changes in the Electrophysiological Properties of Rat Substantia Gelatinosa Neurons
J Neurophysiol,
August 1, 2006;
96(2):
579 - 590.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Lee, M. Tommerdahl, O. V. Favorov, and B. L. Whitsel
Optically Recorded Response of the Superficial Dorsal Horn: Dissociation From Neuronal Activity, Sensitivity to Formalin-Evoked Skin Nociceptor Activation
J Neurophysiol,
July 1, 2005;
94(1):
852 - 864.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. A Graham, A. M Brichta, and R. J Callister
In vivo responses of mouse superficial dorsal horn neurones to both current injection and peripheral cutaneous stimulation
J. Physiol.,
December 15, 2004;
561(3):
749 - 763.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
B. Heinke, R. Ruscheweyh, L. Forsthuber, G. Wunderbaldinger, and J. Sandkuhler
Physiological, neurochemical and morphological properties of a subgroup of GABAergic spinal lamina II neurones identified by expression of green fluorescent protein in mice
J. Physiol.,
October 1, 2004;
560(1):
249 - 266.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Kato, H. Furue, T. Katafuchi, T. Yasaka, Y. Iwamoto, and M. Yoshimura
Electrophysiological mapping of the nociceptive inputs to the substantia gelatinosa in rat horizontal spinal cord slices
J. Physiol.,
October 1, 2004;
560(1):
303 - 315.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|

|