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The Journal of Neuroscience, October 13, 2004, 24(41):9059-9066; doi:10.1523/JNEUROSCI.1645-04.2004

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Cellular/Molecular
Two Interdependent TRPV Channel Subunits, Inactive and Nanchung, Mediate Hearing in Drosophila

Zhefeng Gong,^1 * Wonseok Son,^2 * Yun Doo Chung,^3 * Janghwan Kim,^2,6 Dong Wook Shin,² Colleen A. McClung,¹ Yong Lee,⁴ Hye Won Lee,² Deok-Jin Chang,⁴ Bong-Kiun Kaang,⁴ Hawon Cho,⁵ Uhtaek Oh,⁵ Jay Hirsh,¹ Maurice J. Kernan,³ and Changsoo Kim^2,7

¹Biology Department, University of Virginia, Charlottesville, Virginia 22904, ²Department of Genetics, Hanwha Chemical Company R&D Center, Sinsung-Dong, Yusung-Gu, Daejeon 305-345, Korea, ³Department of Neurobiology and Behavior, State University of New York at Stony Brook, New York 11794, ⁴National Research Laboratory, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea, ⁵Sensory Research Center, Creative Research Initiatives, Seoul National University, Seoul 151-742, Korea, ⁶Department of Microbiology, Chungnam National University, Daejeon 305-764, Korea, and ⁷School of Biological Sciences and Technology, Chonnam National University, Gwangju 500-757, Korea

Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. A functional green fluorescence protein-IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation.

Key words: channel; deafness; Drosophila; hair cell; hearing; mechanosensory

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Received April 29, 2004; revised August 4, 2004; accepted August 4, 2004.

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