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The Journal of Neuroscience, October 27, 2004, 24(43):9469-9474; doi:10.1523/JNEUROSCI.3162-04.2004

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BRIEF COMMUNICATION
Extrasynaptic Localization of Inactivating Calcium-Activated Potassium Channels in Mouse Inner Hair Cells

Sonja J. Pyott,¹ Elisabeth Glowatzki,² James S. Trimmer,³ and Richard W. Aldrich¹

¹Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California 94305-5345, ²Department of Otolaryngology Head and Neck Surgery, The Johns Hopkins School of Medicine, Baltimore, Maryland 21205-2195, and ³Department of Pharmacology, School of Medicine, University of California, Davis, California 95616-8635

Auditory hair cells from nonmammalian vertebrates are electrically tuned to specific sound frequencies primarily by the interactions of voltage-gated calcium channels and calcium-activated potassium (BK) channels colocalized at synaptic active zones. Mammalian inner hair cells are not electrically tuned and, yet, BK channels are also thought to reside at active zones. Using patch-clamp recordings and immunofluorescence, we characterized BK channel expression in mouse inner hair cells. Unexpectedly, these channels have inactivating currents and are clustered near the apex of the cell away from synaptic sites near the base. These results indicate a novel function of BK channels in mammalian inner hair cells and provide a framework for future research.

Key words: slo; KCNMA1; K_Ca1.1; hearing; cochlea; synapse

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Received Aug 2, 2004; revised August 26, 2004; accepted August 27, 2004.

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