QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 27, 2004, 24(43):9513-9520; doi:10.1523/JNEUROSCI.1829-04.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (12) Citing Articles via Google Scholar Google Scholar Articles by Okubo, Y. Articles by Iino, M. Search for Related Content PubMed PubMed Citation Articles by Okubo, Y. Articles by Iino, M.

 Previous Article  |  Next Article 

Cellular/Molecular
Cross Talk between Metabotropic and Ionotropic Glutamate Receptor-Mediated Signaling in Parallel Fiber-Induced Inositol 1,4,5-Trisphosphate Production in Cerebellar Purkinje Cells

Yohei Okubo, Sho Kakizawa, Kenzo Hirose, and Masamitsu Iino

Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan

In many excitatory glutamatergic synapses, both ionotropic glutamate receptors (iGluRs) and metabotropic glutamate receptors (mGluRs) are closely distributed on the postsynaptic membrane. However, the functional significance of the close distribution of the two types of glutamate receptors has not been fully clarified. In this study, we examined the functional interaction between iGluR and mGluR at parallel fiber (PF) Purkinje cell synapses in the generation of inositol 1,4,5-trisphosphate (IP₃), a key second messenger that regulates many important cellular functions. We visualized local IP₃ dynamics in Purkinje cells using the green fluorescent protein-tagged pleckstrin homology domain (GFP-PHD) as a fluorescent IP₃ probe. Purkinje cells were transduced with Sindbis virus encoding GFP-PHD and imaged with a two-photon laser scanning microscope. Translocation of GFP-PHD from the plasma membrane to the cytoplasm attributable to an increase in IP₃ concentration was observed on PF stimulation in fine dendrites of Purkinje cells. Surprisingly, this PF-induced IP₃ production was blocked not only by the group I mGluR antagonist but also by the AMPA receptor (AMPAR) antagonist. The PF-induced IP₃ production was blocked by either the inhibition of G-protein activation by GDP-S or intracellular Ca²⁺ buffering by BAPTA. These results show that IP₃ production is mediated cooperatively by group I mGluR and AMPAR through G-protein activation and Ca²⁺ influx at PF Purkinje cell synapses, identifying the robust cross talk between iGluR and mGluR for the generation of IP₃ signals.

Key words: inositol 1,4,5-trisphosphate; calcium; metabotropic glutamate receptor; AMPA receptor; parallel fiber; Purkinje cells

---

Received May 12, 2004; revised August 3, 2004; accepted August 3, 2004.

This article has been cited by other articles:

				S. J. Kim, Y. Jin, J. Kim, J. H. Shin, P. F. Worley, and D. J. Linden Transient Upregulation of Postsynaptic IP3-Gated Ca Release Underlies Short-Term Potentiation of Metabotropic Glutamate Receptor 1 Signaling in Cerebellar Purkinje Cells J. Neurosci., April 23, 2008; 28(17): 4350 - 4355. [Abstract] [Full Text] [PDF]

				K. Kanemaru, Y. Okubo, K. Hirose, and M. Iino Regulation of Neurite Growth by Spontaneous Ca2+ Oscillations in Astrocytes J. Neurosci., August 15, 2007; 27(33): 8957 - 8966. [Abstract] [Full Text] [PDF]

				D. Billups, B. Billups, R. A. J. Challiss, and S. R. Nahorski Modulation of Gq-Protein-Coupled Inositol Trisphosphate and Ca2+ Signaling by the Membrane Potential J. Neurosci., September 27, 2006; 26(39): 9983 - 9995. [Abstract] [Full Text] [PDF]

				K. Furutani, Y. Okubo, S. Kakizawa, and M. Iino Postsynaptic inositol 1,4,5-trisphosphate signaling maintains presynaptic function of parallel fiber-Purkinje cell synapses via BDNF PNAS, May 30, 2006; 103(22): 8528 - 8533. [Abstract] [Full Text] [PDF]

				M. Canepari and D. Ogden Kinetic, pharmacological and activity-dependent separation of two Ca2+ signalling pathways mediated by type 1 metabotropic glutamate receptors in rat Purkinje neurones J. Physiol., May 15, 2006; 573(1): 65 - 82. [Abstract] [Full Text] [PDF]

				L. F. Horowitz, W. Hirdes, B.-C. Suh, D. W. Hilgemann, K. Mackie, and B. Hille Phospholipase C in Living Cells: Activation, Inhibition, Ca2+ Requirement, and Regulation of M Current J. Gen. Physiol., August 29, 2005; 126(3): 243 - 262. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help