QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 27, 2004, 24(43):9681-9692; doi:10.1523/JNEUROSCI.2800-04.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (15) Citing Articles via Google Scholar Google Scholar Articles by Aradi, I. Articles by Maccaferri, G. Search for Related Content PubMed PubMed Citation Articles by Aradi, I. Articles by Maccaferri, G.

 Previous Article  |  Next Article 

Behavioral/Systems/Cognitive
Cell Type-Specific Synaptic Dynamics of Synchronized Bursting in the Juvenile CA3 Rat Hippocampus

Ildiko Aradi and Gianmaria Maccaferri

Department of Physiology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611

Spontaneous synchronous bursting of the CA3 hippocampus in vitro is a widely studied model of physiological and pathological network synchronization. The role of inhibitory conductances during network bursting is not understood in detail, despite the fact that several antiepileptic drugs target GABA_A receptors. Here, we show that the first manifestation of a burst event is a cell type-specific flurry of GABA_A receptor-mediated inhibitory input to pyramidal cells, but not to stratum oriens horizontal interneurons. Moreover, GABA_A receptor-mediated synaptic input is proportionally smaller in these interneurons compared with pyramidal cells. Computational models and dynamic-clamp studies using experimentally derived conductance waveforms indicate that both these factors modulate spike timing during synchronized activity. In particular, the different kinetics and the larger strength of GABAergic input to pyramidal cells defer action potential initiation and contribute to the observed delay of firing, so that the interneuronal activity leads the burst cycle. In contrast, excitatory inputs to both neuronal populations during a burst are kinetically similar, as required to maintain synchronicity. We also show that the natural pattern of activation of inhibitory and excitatory conductances during a synchronized burst cycle is different within the same neuronal population. In particular, GABA_A receptor-mediated currents activate earlier and outlast the excitatory components driving the bursts. Thus, cell type-specific balance and timing of GABA_A receptor-mediated input are critical to set the appropriate spike timing in pyramidal cells and interneurons and coordinate additional neurotransmitter release modulating burst strength and network frequency.

Key words: interneurons; network; GABA; hippocampus; burst; epilepsy

---

Received July 13, 2004; revised August 27, 2004; accepted September 21, 2004.

This article has been cited by other articles:

				I. Marchionni and G. Maccaferri Quantitative dynamics and spatial profile of perisomatic GABAergic input during epileptiform synchronization in the CA1 hippocampus J. Physiol., December 1, 2009; 587(23): 5691 - 5708. [Abstract] [Full Text] [PDF]

				T. M. Ho, K. A. Pelkey, J. G. Pelletier, R. L. Huganir, J.-C. Lacaille, and C. J. McBain Burst firing induces postsynaptic LTD at developing mossy fibre\#8211;CA3 pyramid synapses J. Physiol., September 15, 2009; 587(18): 4441 - 4454. [Abstract] [Full Text] [PDF]

				J. L. Hellier, D. R. Grosshans, S. J. Coultrap, J. P. Jones, P. Dobelis, M. D. Browning, and K. J. Staley NMDA Receptor Trafficking at Recurrent Synapses Stabilizes the State of the CA3 Network J Neurophysiol, November 1, 2007; 98(5): 2818 - 2826. [Abstract] [Full Text] [PDF]

				J. Wu, Y. Chang, G. Li, F. Xue, J. DeChon, K. Ellsworth, Q. Liu, K. Yang, N. Bahadroani, C. Zheng, et al. Electrophysiological Properties and Subunit Composition of GABAA Receptors in Patients With Gelastic Seizures and Hypothalamic Hamartoma J Neurophysiol, July 1, 2007; 98(1): 5 - 15. [Abstract] [Full Text] [PDF]

				J. Spampanato and I. Mody Spike Timing of Lacunosom-Moleculare Targeting Interneurons and CA3 Pyramidal Cells During High-Frequency Network Oscillations In Vitro J Neurophysiol, July 1, 2007; 98(1): 96 - 104. [Abstract] [Full Text] [PDF]

				V. Zsiros, I. Aradi, and G. Maccaferri Propagation of postsynaptic currents and potentials via gap junctions in GABAergic networks of the rat hippocampus J. Physiol., January 15, 2007; 578(2): 527 - 544. [Abstract] [Full Text] [PDF]

				M. A. Colicos and N. I. Syed Neuronal networks and synaptic plasticity: understanding complex system dynamics by interfacing neurons with silicon technologies J. Exp. Biol., June 15, 2006; 209(12): 2312 - 2319. [Abstract] [Full Text] [PDF]

				J. Ziburkus, J. R. Cressman, E. Barreto, and S. J. Schiff Interneuron and Pyramidal Cell Interplay During In Vitro Seizure-Like Events J Neurophysiol, June 1, 2006; 95(6): 3948 - 3954. [Abstract] [Full Text] [PDF]

				V. Zsiros and G. Maccaferri Electrical Coupling between Interneurons with Different Excitable Properties in the Stratum Lacunosum-Moleculare of the Juvenile CA1 Rat Hippocampus J. Neurosci., September 21, 2005; 25(38): 8686 - 8695. [Abstract] [Full Text] [PDF]

				S. T. Sipila, K. Huttu, I. Soltesz, J. Voipio, and K. Kaila Depolarizing GABA Acts on Intrinsically Bursting Pyramidal Neurons to Drive Giant Depolarizing Potentials in the Immature Hippocampus J. Neurosci., June 1, 2005; 25(22): 5280 - 5289. [Abstract] [Full Text] [PDF]

				G. Maccaferri Stratum oriens horizontal interneurone diversity and hippocampal network dynamics J. Physiol., January 1, 2005; 562(1): 73 - 80. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help