QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 10, 2004, 24(45):10040-10046; doi:10.1523/JNEUROSCI.3643-04.2004

This Article Full Text Full Text (PDF) A correction has been published Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (40) Citing Articles via Google Scholar Google Scholar Articles by Davidson, T. J. Articles by Troy, C. M. Search for Related Content PubMed PubMed Citation Articles by Davidson, T. J. Articles by Troy, C. M.

 Previous Article  |  Next Article 

Cellular/Molecular
Highly Efficient Small Interfering RNA Delivery to Primary Mammalian Neurons Induces MicroRNA-Like Effects before mRNA Degradation

Thomas J. Davidson,¹ Sivan Harel,¹ Valerie A. Arboleda,¹ Giselle F. Prunell,¹ Michael L. Shelanski,^1,3 Lloyd A. Greene,^1,3 and Carol M. Troy^1,2,3

Departments of ¹Pathology and ²Neurology, Taub Institute for the Study of Alzheimer's Disease and the Aging Brain, and ³Center for Neurobiology and Behavior, Columbia University College of Physicians and Surgeons, New York, New York, 10032

The study of protein function in neurons has been hindered by the lack of highly efficient, nontoxic methods of inducing RNA interference in such cells. Here we show that application of synthetic small interfering RNA (siRNA) linked to the vector peptide Penetratin1 results in rapid, highly efficient uptake of siRNA by entire populations of cultured primary mammalian hippocampal and sympathetic neurons. This treatment leads to specific knock-down of targeted proteins within hours without the toxicity associated with transfection. In contrast to current methods, our technique permits study of protein function across entire populations with minimal disturbance of complex cellular networks. Using this technique, we found that protein knock-down (evident after 6 hr) precedes any decrease in targeted message (evident after 24 hr), suggesting an early, translational repression by perfectly targeted siRNAs.

Key words: primary neurons; hippocampal neurons; siRNA; Penetratin1; transduction peptides; RNA interference

---

Received July 19, 2004; accepted September 21, 2004.

This article has been cited by other articles:

				A.-M. Anesti, P. J. Peeters, I. Royaux, and R. S. Coffin Efficient delivery of RNA Interference to peripheral neurons in vivo using herpes simplex virus Nucleic Acids Res., August 1, 2008; 36(14): e86 - e86. [Abstract] [Full Text] [PDF]

				C. J. Parkyn, E. G. M. Vermeulen, R. C. Mootoosamy, C. Sunyach, C. Jacobsen, C. Oxvig, S. Moestrup, Q. Liu, G. Bu, A. Jen, et al. LRP1 controls biosynthetic and endocytic trafficking of neuronal prion protein J. Cell Sci., March 15, 2008; 121(6): 773 - 783. [Abstract] [Full Text] [PDF]

				P. Lundberg, S. El-Andaloussi, T. Sutlu, H. Johansson, and U. Langel Delivery of short interfering RNA using endosomolytic cell-penetrating peptides FASEB J, September 1, 2007; 21(11): 2664 - 2671. [Abstract] [Full Text] [PDF]

				J. Kim, K. Inoue, J. Ishii, W. B. Vanti, S. V. Voronov, E. Murchison, G. Hannon, and A. Abeliovich A MicroRNA Feedback Circuit in Midbrain Dopamine Neurons Science, August 31, 2007; 317(5842): 1220 - 1224. [Abstract] [Full Text] [PDF]

				L. Tonges, P. Lingor, R. Egle, G. P.H. Dietz, A. Fahr, and M. Bahr Stearylated octaarginine and artificial virus-like particles for transfection of siRNA into primary rat neurons RNA, July 1, 2006; 12(7): 1431 - 1438. [Abstract] [Full Text] [PDF]

				H. Paulson RNA interference as potential therapy for neurodegenerative disease: Applications to inclusion-body myositis? Neurology, January 24, 2006; 66(1_suppl_1): S114 - S117. [Abstract] [Full Text] [PDF]

				M. L. Read, S. Singh, Z. Ahmed, M. Stevenson, S. S. Briggs, D. Oupicky, L. B. Barrett, R. Spice, M. Kendall, M. Berry, et al. A versatile reducible polycation-based system for efficient delivery of a broad range of nucleic acids Nucleic Acids Res., May 24, 2005; 33(9): e86 - e86. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help