QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 10, 2004, 24(45):10260-10265; doi:10.1523/JNEUROSCI.1225-04.2004

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (41) Citing Articles via Google Scholar Google Scholar Articles by Kuhn, C. Articles by Meyerhof, W. Search for Related Content PubMed PubMed Citation Articles by Kuhn, C. Articles by Meyerhof, W.

 Previous Article  |  Next Article 

Cellular/Molecular
Bitter Taste Receptors for Saccharin and Acesulfame K

Christina Kuhn,¹ Bernd Bufe,¹ Marcel Winnig,¹ Thomas Hofmann,² Oliver Frank,² Maik Behrens,¹ Tatjana Lewtschenko,² Jay P. Slack,³ Cynthia D. Ward,³ and Wolfgang Meyerhof¹

¹German Institute of Human Nutrition Potsdam-Rehbruecke, Department of Molecular Genetics, 14558 Nuthetal, Germany, ²Institute of Food Chemistry, University of Münster, 48149 Münster, Germany, and ³Givaudan Flavors Corporation, Cincinnati, Ohio 45216

Weight-conscious subjects and diabetics use the sulfonyl amide sweeteners saccharin and acesulfame K to reduce their calorie and sugar intake. However, the intrinsic bitter aftertaste, which is caused by unknown mechanisms, limits the use of these sweeteners. Here, we show by functional expression experiments in human embryonic kidney cells that saccharin and acesulfame K activate two members of the human TAS2R family (hTAS2R43 and hTAS2R44) at concentrations known to stimulate bitter taste. These receptors are expressed in tongue taste papillae. Moreover, the sweet inhibitor lactisole did not block the responses of cells transfected with TAS2R43 and TAS2R44, whereas it did block the response of cells expressing the sweet taste receptor heteromer hTAS1R2-hTAS1R3. The two receptors were also activated by nanomolar concentrations of aristolochic acid, a purely bitter-tasting compound. Thus, hTAS2R43 and hTAS2R44 function as cognate bitter taste receptors and do not contribute to the sweet taste of saccharin and acesulfame K. Consistent with the in vitro data, cross-adaptation studies in human subjects also support the existence of common receptors for both sulfonyl amide sweeteners.

Key words: TAS2R; bitter taste; saccharin; acesulfame K; aristolochic acid; mRNA localization

---

Received April 1, 2004; revised October 1, 2004; accepted October 5, 2004.

This article has been cited by other articles:

				B. D. Gulbransen, T. R. Clapp, T. E. Finger, and S. C. Kinnamon Nasal Solitary Chemoreceptor Cell Responses to Bitter and Trigeminal Stimulants In Vitro J Neurophysiol, June 1, 2008; 99(6): 2929 - 2937. [Abstract] [Full Text] [PDF]

				K. Maehashi, M. Matano, M. Nonaka, S. Udaka, and Y. Yamamoto Riboflavin-Binding Protein Is a Novel Bitter Inhibitor Chem Senses, January 1, 2008; 33(1): 57 - 63. [Abstract] [Full Text] [PDF]

				M. Behrens, S. Foerster, F. Staehler, J.-D. Raguse, and W. Meyerhof Gustatory Expression Pattern of the Human TAS2R Bitter Receptor Gene Family Reveals a Heterogenous Population of Bitter Responsive Taste Receptor Cells J. Neurosci., November 14, 2007; 27(46): 12630 - 12640. [Abstract] [Full Text] [PDF]

				Y. Jiao, S. J. Moon, and C. Montell From the Cover: A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging PNAS, August 28, 2007; 104(35): 14110 - 14115. [Abstract] [Full Text] [PDF]

				C. E. Riera, H. Vogel, S. A. Simon, and J. l. Coutre Artificial sweeteners and salts producing a metallic taste sensation activate TRPV1 receptors Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R626 - R634. [Abstract] [Full Text] [PDF]

				R. K. Palmer The Pharmacology and Signaling of Bitter, Sweet, and Umami Taste Sensing Mol. Interv., April 1, 2007; 7(2): 87 - 98. [Abstract] [Full Text] [PDF]

				R. Yoshida, N. Shigemura, K. Sanematsu, K. Yasumatsu, S. Ishizuka, and Y. Ninomiya Taste Responsiveness of Fungiform Taste Cells With Action Potentials J Neurophysiol, December 1, 2006; 96(6): 3088 - 3095. [Abstract] [Full Text] [PDF]

				N. Rozengurt, S. V. Wu, M. C. Chen, C. Huang, C. Sternini, and E. Rozengurt Colocalization of the {alpha}-subunit of gustducin with PYY and GLP-1 in L cells of human colon Am J Physiol Gastrointest Liver Physiol, November 1, 2006; 291(5): G792 - G802. [Abstract] [Full Text] [PDF]

				M. Behrens, J. Bartelt, C. Reichling, M. Winnig, C. Kuhn, and W. Meyerhof Members of RTP and REEP Gene Families Influence Functional Bitter Taste Receptor Expression J. Biol. Chem., July 21, 2006; 281(29): 20650 - 20659. [Abstract] [Full Text] [PDF]

				A. C. Spector and S. P. Travers The representation of taste quality in the Mammalian nervous system. Behav Cogn Neurosci Rev, September 1, 2005; 4(3): 143 - 191. [Abstract] [PDF]

				S. Rodgers, J. Busch, H. Peters, and E. Christ-Hazelhof Building a Tree of Knowledge: Analysis of Bitter Molecules Chem Senses, September 1, 2005; 30(7): 547 - 557. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help