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The Journal of Neuroscience, December 8, 2004, 24(49):10999-11009; doi:10.1523/JNEUROSCI.3934-04.2004
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Development/Plasticity/Repair
Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination
Yi Zuo,1 *
Jane L. Lubischer,1 *
Hyuno Kang,1 *
Le Tian,1 *
Michelle Mikesh,1
Alexander Marks,4
Virginia L. Scofield,3
Shan Maika,2
Craig Newman,1
Paul Krieg,5 and
Wesley J. Thompson1
1Section of Neurobiology, Institute for Neuroscience, Institute for Cellular and Molecular Biology, and 2Mouse Genetic Engineering Facility, University of Texas, Austin, Texas 78712, 3University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, 4Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, M5G 1L6 Canada, and 5Department of Cell Biology and Anatomy, University of Arizona College of Medicine, Tucson, Arizona 85724
To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.
Key words: neuromuscular junction; Schwann cell; microglia; Langerhans cell; astrocyte; S100 ; S100B; motor neuron; variegation; Bergmann glia
Received Aug 17, 2004;
revised October 29, 2004;
accepted November 3, 2004.
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