 |
The Journal of Neuroscience, December 8, 2004, 24(49):10999-11009; doi:10.1523/JNEUROSCI.3934-04.2004
 Previous Article | Next Article 
Development/Plasticity/Repair
Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark Subsets of Glia, Neurons, Macrophages, and Dendritic Cells for Vital Examination
Yi Zuo,1 *
Jane L. Lubischer,1 *
Hyuno Kang,1 *
Le Tian,1 *
Michelle Mikesh,1
Alexander Marks,4
Virginia L. Scofield,3
Shan Maika,2
Craig Newman,1
Paul Krieg,5 and
Wesley J. Thompson1
1Section of Neurobiology, Institute for Neuroscience, Institute for Cellular and Molecular Biology, and 2Mouse Genetic Engineering Facility, University of Texas, Austin, Texas 78712, 3University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, 4Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, M5G 1L6 Canada, and 5Department of Cell Biology and Anatomy, University of Arizona College of Medicine, Tucson, Arizona 85724
To enable vital observation of glia at the neuromuscular junction, transgenic mice were generated that express proteins of the green fluorescent protein family under control of transcriptional regulatory sequences of the human S100B gene. Terminal Schwann cells were imaged repetitively in living animals of one of the transgenic lines to show that, except for extension and retraction of short processes, the glial coverings of the adult neuromuscular synapse are stable. In other lines, subsets of Schwann cells were labeled. The distribution of label suggests that Schwann cells at individual synapses are clonally related, a finding with implications for how these cells might be sorted during postnatal development. Other labeling patterns, some present in unique lines, included astrocytes, microglia, and subsets of cerebellar Bergmann glia, spinal motor neurons, macrophages, and dendritic cells. We show that lines with labeled macrophages can be used to follow the accumulation of these cells at sites of injury.
Key words: neuromuscular junction; Schwann cell; microglia; Langerhans cell; astrocyte; S100 ; S100B; motor neuron; variegation; Bergmann glia
Received Aug 17, 2004;
revised October 29, 2004;
accepted November 3, 2004.
This article has been cited by other articles:
|
|
|
|
 
F. A. Court, T. H. Gillingwater, S. Melrose, D. L. Sherman, K. N. Greenshields, A. J. Morton, J. B. Harris, H. J. Willison, and R. R. Ribchester
Identity, developmental restriction and reactivity of extralaminar cells capping mammalian neuromuscular junctions
J. Cell Sci.,
December 1, 2008;
121(23):
3901 - 3911.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Barrette, M.-A. Hebert, M. Filali, K. Lafortune, N. Vallieres, G. Gowing, J.-P. Julien, and S. Lacroix
Requirement of Myeloid Cells for Axon Regeneration
J. Neurosci.,
September 17, 2008;
28(38):
9363 - 9376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Song, T. Misgeld, H. Kang, S. Knecht, J. Lu, Y. Cao, S. L. Cotman, D. L. Bishop, and J. W. Lichtman
Lysosomal Activity Associated with Developmental Axon Pruning
J. Neurosci.,
September 3, 2008;
28(36):
8993 - 9001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. M. Vock, O. N. Ponomareva, and M. Rimer
Evidence for Muscle-Dependent Neuromuscular Synaptic Site Determination in Mammals
J. Neurosci.,
March 19, 2008;
28(12):
3123 - 3130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Cahoy, B. Emery, A. Kaushal, L. C. Foo, J. L. Zamanian, K. S. Christopherson, Y. Xing, J. L. Lubischer, P. A. Krieg, S. A. Krupenko, et al.
A Transcriptome Database for Astrocytes, Neurons, and Oligodendrocytes: A New Resource for Understanding Brain Development and Function
J. Neurosci.,
January 2, 2008;
28(1):
264 - 278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Nestor, L.-P. Mok, M. E. Tulapurkar, and S. M. Thompson
Plasticity of Neuron-Glial Interactions Mediated by Astrocytic EphARs
J. Neurosci.,
November 21, 2007;
27(47):
12817 - 12828.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Djukic, K. B. Casper, B. D. Philpot, L.-S. Chin, and K. D. McCarthy
Conditional Knock-Out of Kir4.1 Leads to Glial Membrane Depolarization, Inhibition of Potassium and Glutamate Uptake, and Enhanced Short-Term Synaptic Potentiation
J. Neurosci.,
October 17, 2007;
27(42):
11354 - 11365.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kang, L. Tian, Y.-J. Son, Y. Zuo, D. Procaccino, F. Love, C. Hayworth, J. Trachtenberg, M. Mikesh, L. Sutton, et al.
Regulation of the Intermediate Filament Protein Nestin at Rodent Neuromuscular Junctions by Innervation and Activity
J. Neurosci.,
May 30, 2007;
27(22):
5948 - 5957.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. McCann, Q. T. Nguyen, H. Santo Neto, and J. W. Lichtman
Rapid Synapse Elimination after Postsynaptic Protein Synthesis Inhibition In Vivo
J. Neurosci.,
May 30, 2007;
27(22):
6064 - 6067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Itakura, K. Odaira, K. Yokoyama, M. Osuna, T. Hara, and K. Inoue
Generation of Transgenic Rats Expressing Green Fluorescent Protein in S-100{beta}-Producing Pituitary Folliculo-Stellate Cells and Brain Astrocytes
Endocrinology,
April 1, 2007;
148(4):
1518 - 1523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. R. Hayworth, S. E. Moody, L. A. Chodosh, P. Krieg, M. Rimer, and W. J. Thompson
Induction of neuregulin signaling in mouse schwann cells in vivo mimics responses to denervation.
J. Neurosci.,
June 21, 2006;
26(25):
6873 - 6884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
