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The Journal of Neuroscience, February 18, 2004, 24(7):1719-1725; doi:10.1523/JNEUROSCI.3548-02.2004

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Cellular/Molecular
Early Expression of Sodium Channel Transcripts and Sodium Current by Cajal-Retzius Cells in the Preplate of the Embryonic Mouse Neocortex

Mireille Albrieux,^1,2 Jean-Claude Platel,² Alain Dupuis,² Michel Villaz,² and William J. Moody¹

¹Department of Biology, University of Washington, Seattle, Washington 98195, and ²Canaux Ioniques et Signalisation, Institut National de la Santéet de la Recherche Médicale E9931, Département Réponses et Dynamique Cellulaires, 38054 Grenoble, France

In mouse, the first neurons are generated at embryonic day (E) 12 and form the preplate (PP), which contains a mix of future marginal zone cells, including Cajal-Retzius cells, and subplate cells. To detect developmental changes in channel populations in these earliest-generated neurons of the cerebral cortex, we studied the electrophysiological properties of proliferative cells of the ventricular zone and postmitotic neurons of the PP at E12 and E13, using whole-cell patch-clamp recordings. We found an inward sodium current in 55% of PP cells. To determine whether sodium currents occur in a specific cell type, we stained recorded cells with an antibody for calretinin, a calcium-binding protein found specifically in Cajal-Retzius cells. All calretinin-positive cells had sodium currents, although so did some calretinin-negative cells. To correlate the Na current expression to Na channel gene expression with the Cajal-Retzius cell phenotype, we performed single-cell reverse transcription-PCR on patch-clamp recorded cells to detect expression of the Cajal-Retzius cell marker reelin and the Na channel isoforms SCN 1, 2, and 3. These results showed that virtually all Cajal-Retzius cells (97%), as judged by reelin expression, express the SCN transcript identified as the SCN3 isoform. Of these, 41% presented a functional Na current. There is, however, a substantial SCN-positive population in the PP (27% of SCN-positive cells) that does not express reelin. These results raise the possibility that populations of pioneer neurons of the PP, including Cajal-Retzius cells, gain neuronal physiological properties early in development via expression of the Na_v1.3 (SCN3) Na channel isoform.

Key words: Na current; Cajal-Retzius; reelin; preplate; single-cell RT-PCR; patch clamp

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Received Dec 12, 2002; revised December 4, 2003; accepted December 8, 2003.

This article has been cited by other articles:

				K. Kirmse, A. Dvorzhak, C. Henneberger, R. Grantyn, and S. Kirischuk Cajal Retzius cells in the mouse neocortex receive two types of pre- and postsynaptically distinct GABAergic inputs J. Physiol., December 15, 2007; 585(3): 881 - 895. [Abstract] [Full Text] [PDF]

				K. A. Trzaska, E. V. Kuzhikandathil, and P. Rameshwar Specification of a Dopaminergic Phenotype from Adult Human Mesenchymal Stem Cells Stem Cells, November 1, 2007; 25(11): 2797 - 2808. [Abstract] [Full Text] [PDF]

				J.-C. Platel, S. Boisseau, A. Dupuis, J. Brocard, A. Poupard, M. Savasta, M. Villaz, and M. Albrieux Na+ channel-mediated Ca2+ entry leads to glutamate secretion in mouse neocortical preplate PNAS, December 27, 2005; 102(52): 19174 - 19179. [Abstract] [Full Text] [PDF]

				W. J. Moody and M. M. Bosma Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells Physiol Rev, July 1, 2005; 85(3): 883 - 941. [Abstract] [Full Text] [PDF]

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