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The Journal of Neuroscience, February 25, 2004, 24(8):2054-2064; doi:10.1523/JNEUROSCI.5066-03.2004

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 Previous Article

Cellular/Molecular
The Number of Glutamate Receptors Opened by Synaptic Stimulation in Single Hippocampal Spines

Esther A. Nimchinsky, Ryohei Yasuda, Thomas G. Oertner, and Karel Svoboda

Howard Hughes Medical Institute, The Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

The number of receptors opening after glutamate release is critical for understanding the sources of noise and the dynamic range of synaptic transmission. We imaged [Ca2+] transients mediated by synaptically activated NMDA receptors (NMDA-Rs) in individual spines in rat brain slices. We show that Ca2+ influx through single NMDA-Rs can be reliably detected, allowing us to estimate the number of receptors opening after synaptic transmission. This number is small: at the peak of the synaptic response, less than one NMDA-R is open, on average. Therefore, stochastic interactions between transmitter and receptor contribute substantially to synaptic noise, and glutamate occupies a small fraction of receptors. The number of receptors opening did not scale with spine volume, and smaller spines experience larger [Ca2+] transients during synaptic transmission. Our measurements further demonstrate that optical recordings can be used to study single receptors in intact systems.

Key words: NMDA receptor; synaptic transmission; calcium imaging; 2-photon; saturation

Received Nov 15, 2003; revised January 6, 2004; accepted January 12, 2004.




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