 |
||||
|
||||
|
||||
|
||||
The Journal of Neuroscience, January 5, 2005, 25(1):199-207; doi:10.1523/JNEUROSCI.3861-04.2005
Previous Article | Next Article
Cellular/Molecular
Mechanisms Underlying Developmental Speeding in AMPA-EPSC Decay Time at the Calyx of Held
Maki Koike-Tani, Naoto Saitoh, andTomoyuki Takahashi Department of Neurophysiology, University of Tokyo Graduate School of Medicine, Tokyo 113-0033, Japan
The time course of synaptic conductance is important in temporal precision of information processing in the neuronal network. The AMPA receptor (AMPAR)-mediated EPSCs at the calyx of Held become faster in decay time as animals mature. To clarify how desensitization and deactivation of AMPARs contribute to developmental speeding of EPSCs, we compared the decay time of quantal EPSCs (qEPSCs) with the deactivation and desensitization times of AMPAR currents induced in excised patches by fast glutamate application (AMPA patch currents). Both the deactivation and desensitization times of AMPA patch currents became markedly faster from postnatal day 7 (P7) to P14 and changed little thereafter. In individual neurons, throughout development (P7-P21), the time constants of deactivation and fast desensitization in AMPA patch currents were similar to each other and close to the qEPSC decay time constant. Cyclothiazide (CTZ) abolished the fast desensitization, prolonged deactivation of AMPA patch currents, and slowed the decay time of EPSCs. The effects of CTZ on AMPA patch currents were unchanged throughout development, whereas its effect on EPSCs became weaker as animals matured. In single-cell reverse transcription-PCR analysis, glutamate receptor subunit 4 (GluR4) flop increased from P7 to P14 and changed little thereafter. At P7, the GluR4 flop abundance had an inverse correlation with the qEPSC decay time. These results together suggest that both desensitization and deactivation of AMPARs are involved in the EPSC decay time, but the contribution of desensitization decreases during postnatal development at the calyx of Held.
Key words: EPSC; AMPA receptor; desensitization; deactivation; postnatal development; single-cell RT-PCR
Received Sep 17, 2004; revised November 18, 2004; accepted November 18, 2004.
This article has been cited by other articles:
M. Koike-Tani, T. Kanda, N. Saitoh, T. Yamashita, and T. Takahashi
Involvement of AMPA receptor desensitization in short-term synaptic depression at the calyx of Held in developing rats
J. Physiol., May 1, 2008; 586(9): 2263 - 2275.
[Abstract] [Full Text] [PDF]
G. Gonzalez-Burgos, S. Kroener, A. V. Zaitsev, N. V. Povysheva, L. S. Krimer, G. Barrionuevo, and D. A. Lewis
Functional Maturation of Excitatory Synapses in Layer 3 Pyramidal Neurons during Postnatal Development of the Primate Prefrontal Cortex
Cereb Cortex, March 1, 2008; 18(3): 626 - 637.
[Abstract] [Full Text] [PDF]
A. J. Milnerwood and L. A. Raymond
Corticostriatal synaptic function in mouse models of Huntington's disease: early effects of huntingtin repeat length and protein load
J. Physiol., December 15, 2007; 585(3): 817 - 831.
[Abstract] [Full Text] [PDF]
Y. Nakamura and T. Takahashi
Developmental changes in potassium currents at the rat calyx of Held presynaptic terminal
J. Physiol., June 15, 2007; 581(3): 1101 - 1112.
[Abstract] [Full Text] [PDF]
X.-S. Wu, L. Xue, R. Mohan, K. Paradiso, K. D. Gillis, and L.-G. Wu
The Origin of Quantal Size Variation: Vesicular Glutamate Concentration Plays a Significant Role
J. Neurosci., March 14, 2007; 27(11): 3046 - 3056.
[Abstract] [Full Text] [PDF]
N. E. Schoppa
AMPA/Kainate Receptors Drive Rapid Output and Precise Synchrony in Olfactory Bulb Granule Cells
J. Neurosci., December 13, 2006; 26(50): 12996 - 13006.
[Abstract] [Full Text] [PDF]
Y.-M. Yang and L.-Y. Wang
Amplitude and Kinetics of Action Potential-Evoked Ca2+ Current and Its Efficacy in Triggering Transmitter Release at the Developing Calyx of Held Synapse
J. Neurosci., May 24, 2006; 26(21): 5698 - 5708.
[Abstract] [Full Text] [PDF]
M. Youssoufian, S. Oleskevich, and B. Walmsley
Development of a Robust Central Auditory Synapse in Congenital Deafness
J Neurophysiol, November 1, 2005; 94(5): 3168 - 3180.
[Abstract] [Full Text] [PDF]
H. Taschenberger, V. Scheuss, and E. Neher
Release kinetics, quantal parameters and their modulation during short-term depression at a developing synapse in the rat CNS
J. Physiol., October 15, 2005; 568(2): 513 - 537.
[Abstract] [Full Text] [PDF]
R. Renden, H. Taschenberger, N. Puente, D. A. Rusakov, R. Duvoisin, L.-Y. Wang, K. P. Lehre, and H. von Gersdorff
Glutamate Transporter Studies Reveal the Pruning of Metabotropic Glutamate Receptors and Absence of AMPA Receptor Desensitization at Mature Calyx of Held Synapses
J. Neurosci., September 14, 2005; 25(37): 8482 - 8497.
[Abstract] [Full Text] [PDF]
C. L. Palmer, L. Cotton, and J. M. Henley
The Molecular Pharmacology and Cell Biology of {alpha}-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Receptors
Pharmacol. Rev., June 1, 2005; 57(2): 253 - 277.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|