QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, January 5, 2005, 25(1):96-107; doi:10.1523/JNEUROSCI.3748-04.2005

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (37) Citing Articles via Google Scholar Google Scholar Articles by Collin, T. Articles by Llano, I. Search for Related Content PubMed PubMed Citation Articles by Collin, T. Articles by Llano, I.

 Previous Article  |  Next Article 

Cellular/Molecular
Developmental Changes in Parvalbumin Regulate Presynaptic Ca²⁺ Signaling

Thibault Collin,¹ Mireille Chat,¹ Marie Gabrielle Lucas,¹ Herman Moreno,² Peter Racay,^3,4 Beat Schwaller,² Alain Marty,¹ and Isabel Llano¹

¹Laboratory of Cerebral Physiology, Centre National de la Recherche Scientifique, University Paris 5, 75006 Paris, France, ²Gertrude H. Sergievsky and Taub Center for Alzheimer Research, Columbia University, New York, New York 10032, ³Division of Histology, Department of Medicine, University of Fribourg, CH1705 Fribourg, Switzerland, and ⁴Jessenius Faculty of Medicine, Institute of Biochemistry, Comenius University, SK-03601 Martin, Slovak Republic

Certain interneurons contain large concentrations of specific Ca²⁺-binding proteins (CBPs), but consequences on presynaptic Ca²⁺ signaling are poorly understood. Here we show that expression of the slow CBP parvalbumin (PV) in cerebellar interneurons is cell specific and developmentally regulated, leading to characteristic changes in presynaptic Ca²⁺ dynamics (Ca_i). Using whole-cell recording and fluorescence imaging, we studied action potential-evoked Ca_i transients in axons of GABA-releasing interneurons from mouse cerebellum. At early developmental stages [postnatal days 10-12 (P10-P12)], decay kinetics were significantly faster for basket cells than for stellate cells, whereas at P19-P21 both interneurons displayed fast decay kinetics. Biochemical and immunocytochemical analysis showed parallel changes in the expression levels and cellular distribution of PV. By comparing wild-type and PV(-/-) mice, PV was shown to accelerate the initial decay of action potential-evoked Ca_i signals in single varicosities and to introduce an additional slow phase that summates during bursts of action potentials. The fast initial Ca_i decay accounts for a previous report that PV elimination favors synaptic facilitation. The slow decay component is responsible for a pronounced, PV-dependent, delayed transmitter release that we describe here at interneuron-interneuron synapses after presynaptic bursts of action potentials. Numerical simulations account for the effect of PV on Ca_i kinetics, allow estimates for the axonal PV concentration (150 µM), and predict the time course of volume-averaged Ca_i in the absence of exogenous buffer. Overall, PV arises as a major contributor to presynaptic Ca_i signals and synaptic integration in the cerebellar cortex.

Key words: calcium; synapses; cerebellum; patch clamp; imaging; development

---

Received Sep 9, 2004; revised November 10, 2004; accepted November 11, 2004.

This article has been cited by other articles:

				V. Egger and O. Stroh Calcium buffering in rodent olfactory bulb granule cells and mitral cells J. Physiol., September 15, 2009; 587(18): 4467 - 4479. [Abstract] [Full Text] [PDF]

				T. Collin, R. Franconville, B. E. Ehrlich, and I. Llano Activation of Metabotropic Glutamate Receptors Induces Periodic Burst Firing and Concomitant Cytosolic Ca2+ Oscillations in Cerebellar Interneurons J. Neurosci., July 22, 2009; 29(29): 9281 - 9291. [Abstract] [Full Text] [PDF]

				B. Rossi, G. Maton, and T. Collin Calcium-permeable presynaptic AMPA receptors in cerebellar molecular layer interneurones J. Physiol., November 1, 2008; 586(21): 5129 - 5145. [Abstract] [Full Text] [PDF]

				G. Gonzalez-Burgos and D. A. Lewis GABA Neurons and the Mechanisms of Network Oscillations: Implications for Understanding Cortical Dysfunction in Schizophrenia Schizophr Bull, September 1, 2008; 34(5): 944 - 961. [Abstract] [Full Text] [PDF]

				Y. Aponte, J. Bischofberger, and P. Jonas Efficient Ca2+ buffering in fast-spiking basket cells of rat hippocampus J. Physiol., April 15, 2008; 586(8): 2061 - 2075. [Abstract] [Full Text] [PDF]

				M. C. Harrisingh, Y. Wu, G. A. Lnenicka, and M. N. Nitabach Intracellular Ca2+ Regulates Free-Running Circadian Clock Oscillation In Vivo J. Neurosci., November 14, 2007; 27(46): 12489 - 12499. [Abstract] [Full Text] [PDF]

				D. Orduz and I. Llano Recurrent axon collaterals underlie facilitating synapses between cerebellar Purkinje cells PNAS, November 6, 2007; 104(45): 17831 - 17836. [Abstract] [Full Text] [PDF]

				Y. Ben-Ari, J.-L. Gaiarsa, R. Tyzio, and R. Khazipov GABA: A Pioneer Transmitter That Excites Immature Neurons and Generates Primitive Oscillations Physiol Rev, October 1, 2007; 87(4): 1215 - 1284. [Abstract] [Full Text] [PDF]

				M. Muller, F. Felmy, B. Schwaller, and R. Schneggenburger Parvalbumin Is a Mobile Presynaptic Ca2+ Buffer in the Calyx of Held that Accelerates the Decay of Ca2+ and Short-Term Facilitation J. Neurosci., February 28, 2007; 27(9): 2261 - 2271. [Abstract] [Full Text] [PDF]

				T. P. Jensen, A. G. Filoteo, T. Knopfel, and R. M. Empson Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3 J. Physiol., February 15, 2007; 579(1): 85 - 99. [Abstract] [Full Text] [PDF]

				R. L. P. Habets and J. G. G. Borst An Increase in Calcium Influx Contributes to Post-Tetanic Potentiation at the Rat Calyx of Held Synapse J Neurophysiol, December 1, 2006; 96(6): 2868 - 2876. [Abstract] [Full Text] [PDF]

				S. Mejia-Gervacio and A. Marty Control of interneurone firing pattern by axonal autoreceptors in the juvenile rat cerebellum J. Physiol., February 15, 2006; 571(1): 43 - 55. [Abstract] [Full Text] [PDF]

				J. W. Kinney, C. N. Davis, I. Tabarean, B. Conti, T. Bartfai, and M. M. Behrens A Specific Role for NR2A-Containing NMDA Receptors in the Maintenance of Parvalbumin and GAD67 Immunoreactivity in Cultured Interneurons J. Neurosci., February 1, 2006; 26(5): 1604 - 1615. [Abstract] [Full Text] [PDF]

				D. A. Rusakov, F. Saitow, K. P. Lehre, and S. Konishi Modulation of Presynaptic Ca2+ Entry by AMPA Receptors at Individual GABAergic Synapses in the Cerebellum J. Neurosci., May 18, 2005; 25(20): 4930 - 4940. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help