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The Journal of Neuroscience, April 13, 2005, 25(15):3787-3792; doi:10.1523/JNEUROSCI.5312-04.2005

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Constitutively Active G-Protein-Gated Inwardly Rectifying K⁺ Channels in Dendrites of Hippocampal CA1 Pyramidal Neurons

Xixi Chen and Daniel Johnston

Department of Neuroscience, Baylor College of Medicine, Houston, Texas 77030, and Marine Biology Laboratory, Woods Hole, Massachusetts 02543

A diversity of ion channels contributes to the active properties of neuronal dendrites. From the apical dendrites of hippocampal CA1 pyramidal neurons, we recorded inwardly rectifying K⁺ channels with a single-channel conductance of 33 pS. The inwardly rectifying K⁺ channels were constitutively active at the resting membrane potential. The amount of constitutive channel activity was significantly larger in the apical dendrites than in the soma. Activities of these inwardly rectifying K⁺ channels were inhibited by Ba²⁺ (200 µM) and tertiapin (10 nM), both of which are believed to block G-protein-coupled inwardly rectifying K⁺ (GIRK) channels. Intracellularly applied GTPS (20 µM) during dual dendritic recordings significantly increased constitutive channel activity. Baclofen (20 µM), an agonist for the G-protein-coupled GABA_B receptor, also significantly increased the level of channel activity. Therefore, these channels are GIRK channels, which are constitutively active at rest in the apical dendrites of CA1 pyramidal neurons and can be further activated via G-protein-coupled neurotransmitter receptors.

Key words: K⁺ channels; inward rectifier; hippocampus; dendrites; G-protein-coupled receptors; single-channel analysis

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Received Oct 20, 2004; revised March 1, 2005; accepted March 2, 2005.

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