 |
||||
|
||||
|
||||
|
||||
|
||||
The Journal of Neuroscience, June 1, 2005, 25(22):5404-5412; doi:10.1523/JNEUROSCI.1039-05.2005
Previous Article | Next Article
Cellular/Molecular
Odorant-Induced Activation of Extracellular Signal-Regulated Kinase/Mitogen-Activated Protein Kinase in the Olfactory Bulb Promotes Survival of Newly Formed Granule Cells
Naofumi Miwa andDaniel R. Storm Department of Pharmacology, University of Washington, Seattle, Washington 98195
Extracellular signal-regulated kinase 1/2 (Erk1/2)/mitogen-activated protein (MAP) kinase (MAPK) plays a significant role in neuronal survival, including odorant-induced, activity-dependent survival of olfactory sensory neurons in the main olfactory epithelium. Here, we examined the role of MAPK for the survival of neurons in the olfactory bulb. To study odorant-induced activation of MAPK in the olfactory bulb, mice were exposed to odorants in vivo, and MAPK was assayed. Exposure of mice to some odorants in vivo activated MAPK in granule cells 10 min after exposure. Activation of MAPK was particularly evident in the nucleus and dendrites of granule cells. Because MAPK activation can augment neuronal survival, odorant enhancement of granule cell survival was monitored by bromodeoxyuridine (BrdU) incorporation. Long-term exposure to odorants increased the survival of newly formed granule cells as well as the number of granule cells that were both BrdU+ and phospho-Erk+. Inhibition of MAPK by administration of SL327 in vivo blocked the odorant-induced increase in newly formed granule cells, suggesting that activation of MAPK promotes the survival of granule cells in the olfactory bulb. Studies using cultured granule cells confirmed that activation of MAPK in granule cells protects them against strong apoptotic signals. These data suggest that stimulation of MAPK in olfactory bulb granule cells by some odorants may contribute to the survival of newly formed granule cells caused by odorant exposure.
Key words: Erk1/2; olfactory bulb; granule cell; BrdU; survival; odorants
Received Nov 4, 2004; revised April 20, 2005; accepted April 25, 2005.
This article has been cited by other articles:
A. Mouret, G. Gheusi, M.-M. Gabellec, F. de Chaumont, J.-C. Olivo-Marin, and P.-M. Lledo
Learning and Survival of Newly Generated Neurons: When Time Matters
J. Neurosci., November 5, 2008; 28(45): 11511 - 11516.
[Abstract] [Full Text] [PDF]
M. Alonso, I. Ortega-Perez, M. S. Grubb, J.-P. Bourgeois, P. Charneau, and P.-M. Lledo
Turning Astrocytes from the Rostral Migratory Stream into Neurons: A Role for the Olfactory Sensory Organ
J. Neurosci., October 22, 2008; 28(43): 11089 - 11102.
[Abstract] [Full Text] [PDF]
G. Gheusi and P.-M. Lledo
Control of Early Events in Olfactory Processing by Adult Neurogenesis
Chem Senses, May 1, 2007; 32(4): 397 - 409.
[Abstract] [Full Text] [PDF]
E. Gascon, L. Vutskits, B. Jenny, P. Durbec, and J. Z. Kiss
PSA-NCAM in postnatally generated immature neurons of the olfactory bulb: a crucial role in regulating p75 expression and cell survival
Development, March 15, 2007; 134(6): 1181 - 1190.
[Abstract] [Full Text] [PDF]
S. S. P. Magavi, B. D. Mitchell, O. Szentirmai, B. S. Carter, and J. D. Macklis
Adult-Born and Preexisting Olfactory Granule Neurons Undergo Distinct Experience-Dependent Modifications of their Olfactory Responses In Vivo
J. Neurosci., November 16, 2005; 25(46): 10729 - 10739.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|