 |
The Journal of Neuroscience, June 29, 2005, 25(26):6037-6046; doi:10.1523/JNEUROSCI.1221-05.2005
 Previous Article | Next Article 
Cellular/Molecular
NMDA Receptor Subunit-Dependent [Ca2+] Signaling in Individual Hippocampal Dendritic Spines
Aleksander Sobczyk,1,2
Volker Scheuss,1 and
Karel Svoboda1
1Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, and 2Department of Physics, State University of New York at Stony Brook, Stony Brook, New York 11794
Ca2+ influx through synaptic NMDA receptors (NMDA-Rs) triggers a variety of adaptive cellular processes. To probe NMDA-R-mediated [Ca2+] signaling, we used two-photon glutamate uncaging to stimulate NMDA-Rs on individual dendritic spines of CA1 pyramidal neurons in rat brain slices. We measured NMDA-R currents at the soma and NMDA-R-mediated [Ca2+] transients in stimulated spines ( [Ca2+]). Uncaging-evoked NMDA-R current amplitudes were independent of the size of the stimulated spine, implying that smaller spines contain higher densities of functional NMDA-Rs. The ratio of [Ca2+] over NMDA-R current was highly variable (factor of 10) across spines, especially for small spines. These differences were not explained by heterogeneity in spine sizes or diffusional coupling between spines and their parent dendrites. In addition, we find that small spines have NMDA-R currents that are sensitive to NMDA-R NR2B subunit-specific antagonists. With block of NR2B-containing receptors, the range of [Ca2+]/NMDA-R current ratios and their average value were much reduced. Our data suggest that individual spines can regulate the subunit composition of their NMDA-Rs and the effective fractional Ca2+ current through these receptors.
Key words: NMDA receptor; NR2B subunit; dendritic spines; two-photon; [Ca2+] imaging; glutamate uncaging
Received March 29, 2005;
revised May 12, 2005;
accepted May 13, 2005.
This article has been cited by other articles:
|
|
|
|
 
J. de Marchena, A. C. Roberts, P. G. Middlebrooks, V. Valakh, K. Yashiro, L. R. Wilfley, and B. D. Philpot
NMDA Receptor Antagonists Reveal Age-Dependent Differences in the Properties of Visual Cortical Plasticity
J Neurophysiol,
October 1, 2008;
100(4):
1936 - 1948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Xu, M. Weerapura, M. K. Ali, M. F. Jackson, H. Li, G. Lei, S. Xue, C. L. Kwan, M. F. Manolson, K. Yang, et al.
Control of Excitatory Synaptic Transmission by C-terminal Src Kinase
J. Biol. Chem.,
June 20, 2008;
283(25):
17503 - 17514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Sotres-Bayon, L. Diaz-Mataix, D. E. A. Bush, and J. E. LeDoux
Dissociable Roles for the Ventromedial Prefrontal Cortex and Amygdala in Fear Extinction: NR2B Contribution
Cereb Cortex,
June 17, 2008;
(2008)
bhn099v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Miwa, M. Fukaya, A. M. Watabe, M. Watanabe, and T. Manabe
Functional contributions of synaptically localized NR2B subunits of the NMDA receptor to synaptic transmission and long-term potentiation in the adult mouse CNS
J. Physiol.,
May 15, 2008;
586(10):
2539 - 2550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. L. Bloodgood and B. L. Sabatini
Regulation of synaptic signalling by postsynaptic, non-glutamate receptor ion channels
J. Physiol.,
March 15, 2008;
586(6):
1475 - 1480.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Busetto, M. J. Higley, and B. L. Sabatini
Developmental presence and disappearance of postsynaptically silent synapses on dendritic spines of rat layer 2/3 pyramidal neurons
J. Physiol.,
March 15, 2008;
586(6):
1519 - 1527.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Matsuzaki, G. C. R. Ellis-Davies, and H. Kasai
Three-Dimensional Mapping of Unitary Synaptic Connections by Two-Photon Macro Photolysis of Caged Glutamate
J Neurophysiol,
March 1, 2008;
99(3):
1535 - 1544.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Ewald, K. R. Van Keuren-Jensen, C. D. Aizenman, and H. T. Cline
Roles of NR2A and NR2B in the Development of Dendritic Arbor Morphology In Vivo
J. Neurosci.,
January 23, 2008;
28(4):
850 - 861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Z. Harris and D. L. Pettit
Extrasynaptic and synaptic NMDA receptors form stable and uniform pools in rat hippocampal slices
J. Physiol.,
October 15, 2007;
584(2):
509 - 519.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. B. Awatramani, J. D. Boyd, K. R. Delaney, and T. H. Murphy
Effective release rates at single rat Schaffer collateral-CA1 synapses during sustained theta-burst activity revealed by optical imaging
J. Physiol.,
July 15, 2007;
582(2):
583 - 595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. C. R. Ellis-Davies, M. Matsuzaki, M. Paukert, H. Kasai, and D. E. Bergles
4-Carboxymethoxy-5,7-Dinitroindolinyl-Glu: An Improved Caged Glutamate for Expeditious Ultraviolet and Two-Photon Photolysis in Brain Slices
J. Neurosci.,
June 20, 2007;
27(25):
6601 - 6604.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lisman and S. Raghavachari
A Unified Model of the Presynaptic and Postsynaptic Changes During LTP at CA1 Synapses
Sci. Signal.,
October 10, 2006;
2006(356):
re11 - re11.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Scheuss, R. Yasuda, A. Sobczyk, and K. Svoboda
Nonlinear [Ca2+] Signaling in Dendrites and Spines Caused by Activity-Dependent Depression of Ca2+ Extrusion
J. Neurosci.,
August 2, 2006;
26(31):
8183 - 8194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Tsui and R. C. Malenka
Substrate Localization Creates Specificity in Calcium/Calmodulin-dependent Protein Kinase II Signaling at Synapses
J. Biol. Chem.,
May 12, 2006;
281(19):
13794 - 13804.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Calabrese, M. S. Wilson, and S. Halpain
Development and Regulation of Dendritic Spine Synapses
Physiology,
February 1, 2006;
21(1):
38 - 47.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. U. Bayer, E. LeBel, G. L. McDonald, H. O'Leary, H. Schulman, and P. De Koninck
Transition from Reversible to Persistent Binding of CaMKII to Postsynaptic Sites and NR2B
J. Neurosci.,
January 25, 2006;
26(4):
1164 - 1174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
