QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, July 27, 2005, 25(30):6958-6961; doi:10.1523/JNEUROSCI.1058-05.2005

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (21) Citing Articles via Google Scholar Google Scholar Articles by Kim, T. Articles by Loh, Y. P. Search for Related Content PubMed PubMed Citation Articles by Kim, T. Articles by Loh, Y. P.

 Previous Article  |  Next Article 

BRIEF COMMUNICATION
Chromogranin A Deficiency in Transgenic Mice Leads to Aberrant Chromaffin Granule Biogenesis

Taeyoon Kim,¹ Chun-fa Zhang,¹ Ziqing Sun,² Heling Wu,² and Y. Peng Loh¹

¹Section on Cellular Neurobiology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, and ²College of Biology, Peking University, 100871 Beijing, China

The biogenesis of dense-core secretory granules (DCGs), organelles responsible for the storage and secretion of neurotransmitters and neuropeptides in chromaffin cells, is poorly understood. Chromogranin A (CgA), which binds catecholamines for storage in the lumen of chromaffin granules, has been shown to be involved in DCG biogenesis in neuroendocrine PC12 cells. Here, we report that downregulation of CgA expression in vivo by expressing antisense RNA against CgA in transgenic mice led to a significant reduction in DCG formation in adrenal chromaffin cells. The number of DCGs formed in CgA antisense transgenic mice was directly correlated with the amount of CgA present in adrenal medulla. In addition, DCGs showed an increase in size, with enlargement in the volume around the dense core, a phenomenon that occurs to maintain constant "free" catecholamine concentration in the lumen of these granules. The extent of DCG swelling was inversely correlated with the number of DCGs formed, as well as the amount of CgA present in the adrenal glands of CgA antisense transgenic mice. These data indicate an essential role of CgA in regulating chromaffin DCG biogenesis and catecholamine storage in vivo.

Key words: chromogranin A; dense-core; secretory granule; biogenesis; transgenic; chromaffin; catecholamine; adrenal medulla

---

Received March 18, 2005; revised May 24, 2005; accepted June 17, 2005.

This article has been cited by other articles:

				N. Biswas, J. L. Rodriguez-Flores, M. Courel, J. R. Gayen, S. M. Vaingankar, M. Mahata, J. W. Torpey, L. Taupenot, D. T. O'Connor, and S. K. Mahata Cathepsin L Colocalizes with Chromogranin A in Chromaffin Vesicles to Generate Active Peptides Endocrinology, August 1, 2009; 150(8): 3547 - 3557. [Abstract] [Full Text] [PDF]

				M. Montero-Hadjadje, S. Elias, L. Chevalier, M. Benard, Y. Tanguy, V. Turquier, L. Galas, L. Yon, M. M. Malagon, A. Driouich, et al. Chromogranin A Promotes Peptide Hormone Sorting to Mobile Granules in Constitutively and Regulated Secreting Cells: ROLE OF CONSERVED N- AND C-TERMINAL PEPTIDES J. Biol. Chem., May 1, 2009; 284(18): 12420 - 12431. [Abstract] [Full Text] [PDF]

				N. R. Mahapatra Catestatin is a novel endogenous peptide that regulates cardiac function and blood pressure Cardiovasc Res, December 1, 2008; 80(3): 330 - 338. [Abstract] [Full Text] [PDF]

				L. Han, M. Suda, K. Tsuzuki, R. Wang, Y. Ohe, H. Hirai, T. Watanabe, T. Takeuchi, and M. Hosaka A Large Form of Secretogranin III Functions as a Sorting Receptor for Chromogranin A Aggregates in PC12 Cells Mol. Endocrinol., August 1, 2008; 22(8): 1935 - 1949. [Abstract] [Full Text] [PDF]

				M. Courel, C. Rodemer, S. T. Nguyen, A. Pance, A. P. Jackson, D. T. O'Connor, and L. Taupenot Secretory Granule Biogenesis in Sympathoadrenal Cells: IDENTIFICATION OF A GRANULOGENIC DETERMINANT IN THE SECRETORY PROHORMONE CHROMOGRANIN A J. Biol. Chem., December 8, 2006; 281(49): 38038 - 38051. [Abstract] [Full Text] [PDF]

				G. N. Hendy, T. Li, M. Girard, R. C. Feldstein, S. Mulay, R. Desjardins, R. Day, A. C. Karaplis, M. L. Tremblay, and L. Canaff Targeted Ablation of the Chromogranin A (Chga) Gene: Normal Neuroendocrine Dense-Core Secretory Granules and Increased Expression of Other Granins Mol. Endocrinol., August 1, 2006; 20(8): 1935 - 1947. [Abstract] [Full Text] [PDF]

				T. Kim, M. C. Gondre-Lewis, I. Arnaoutova, and Y. P. Loh Dense-Core Secretory Granule Biogenesis Physiology, April 1, 2006; 21(2): 124 - 133. [Abstract] [Full Text] [PDF]

				T. Kim and Y. P. Loh Protease Nexin-1 Promotes Secretory Granule Biogenesis by Preventing Granule Protein Degradation Mol. Biol. Cell, February 1, 2006; 17(2): 789 - 798. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help