QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, August 3, 2005, 25(31):7150-7158; doi:10.1523/JNEUROSCI.0683-05.2005

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (33) Citing Articles via Google Scholar Google Scholar Articles by Corvetti, L. Articles by Rossi, F. Search for Related Content PubMed PubMed Citation Articles by Corvetti, L. Articles by Rossi, F.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Degradation of Chondroitin Sulfate Proteoglycans Induces Sprouting of Intact Purkinje Axons in the Cerebellum of the Adult Rat

Luigi Corvetti and Ferdinando Rossi

Department of Neuroscience and Rita Levi Montalcini Center for Brain Repair, University of Turin, I-10125 Turin, Italy

Chondroitin sulfate proteoglycans are major constituents of the extracellular matrix and form perineuronal nets. Information regarding the growth-inhibitory activity of these molecules after injury is rapidly expanding. However, less is known about their physiological role in the adult undamaged CNS. Here, we investigated the function of chondroitin sulfate proteoglycans in maintaining the proper structure of Purkinje axons in the cerebellum of adult rats. To this end, we examined the morphology and distribution of intracortical Purkinje neurites after intraparenchymal injection of chondroitinase ABC. Staining with the lectin Wisteria floribunda agglutinin or 2B6 antibodies showed that this treatment efficiently removed chondroitin sulfate proteoglycans from wide areas of the cerebellar cortex. In the same sites, there was a profuse outgrowth of terminal branches from the Purkinje infraganglionic plexus, which invaded the deeper regions of the granular layer. In contrast, myelinated axon segments were not affected and maintained their normal relationship with oligodendroglial sheaths. Purkinje axon sprouting was first evident at 4 d and increased further at 7 d after enzyme application. Within 42 d, the expression pattern of chondroitin sulfate proteoglycans gradually recovered, whereas axonal modifications progressively regressed. Our results show that, in the absence of injury or novel external stimuli, degradation of chondroitin sulfate proteoglycans is sufficient to induce Purkinje axon sprouting but not the formation of long-lasting synaptic contacts. Together with other growth-inhibitory molecules, such as myelin-associated proteins, chondroitin sulfate proteoglycans restrict structural plasticity of intact Purkinje axons to maintain normal wiring patterns in the adult cerebellar cortex.

Key words: axon growth inhibitory molecules; axon regeneration; structural plasticity; extracellular matrix; myelin; Purkinje cell

---

Received Feb 19, 2005; revised June 16, 2005; accepted June 17, 2005.

This article has been cited by other articles:

				M. Ishii and N. Maeda Oversulfated Chondroitin Sulfate Plays Critical Roles in the Neuronal Migration in the Cerebral Cortex J. Biol. Chem., November 21, 2008; 283(47): 32610 - 32620. [Abstract] [Full Text] [PDF]

				M. Ishii and N. Maeda Spatiotemporal expression of chondroitin sulfate sulfotransferases in the postnatal developing mouse cerebellum Glycobiology, August 1, 2008; 18(8): 602 - 614. [Abstract] [Full Text] [PDF]

				Y. Sato, K. Nakanishi, M. Hayakawa, H. Kakizawa, A. Saito, Y. Kuroda, M. Ida, Y. Tokita, S. Aono, F. Matsui, et al. Reduction of Brain Injury in Neonatal Hypoxic--Ischemic Rats by Intracerebroventricular Injection of Neural Stem/Progenitor Cells Together With Chondroitinase ABC Reproductive Sciences, July 1, 2008; 15(6): 613 - 620. [Abstract] [PDF]

				C. M. Galtrey, R. A. Asher, F. Nothias, and J. W. Fawcett Promoting plasticity in the spinal cord with chondroitinase improves functional recovery after peripheral nerve repair Brain, April 1, 2007; 130(4): 926 - 939. [Abstract] [Full Text] [PDF]

				A. W. Barritt, M. Davies, F. Marchand, R. Hartley, J. Grist, P. Yip, S. B. McMahon, and E. J. Bradbury Chondroitinase ABC Promotes Sprouting of Intact and Injured Spinal Systems after Spinal Cord Injury. J. Neurosci., October 18, 2006; 26(42): 10856 - 10867. [Abstract] [Full Text] [PDF]

				S. S. Deepa, D. Carulli, C. Galtrey, K. Rhodes, J. Fukuda, T. Mikami, K. Sugahara, and J. W. Fawcett Composition of Perineuronal Net Extracellular Matrix in Rat Brain: A DIFFERENT DISACCHARIDE COMPOSITION FOR THE NET-ASSOCIATED PROTEOGLYCANS J. Biol. Chem., June 30, 2006; 281(26): 17789 - 17800. [Abstract] [Full Text] [PDF]

				J. M. Massey, C. H. Hubscher, M. R. Wagoner, J. A. Decker, J. Amps, J. Silver, and S. M. Onifer Chondroitinase ABC digestion of the perineuronal net promotes functional collateral sprouting in the cuneate nucleus after cervical spinal cord injury. J. Neurosci., April 19, 2006; 26(16): 4406 - 4414. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help