QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, August 24, 2005, 25(34):7821-7830; doi:10.1523/JNEUROSCI.1790-05.2005

This Article Full Text Full Text (PDF) Supplemental Material Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (23) Citing Articles via Google Scholar Google Scholar Articles by Kvachnina, E. Articles by Ponimaskin, E. G. Search for Related Content PubMed PubMed Citation Articles by Kvachnina, E. Articles by Ponimaskin, E. G.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
5-HT₇ Receptor Is Coupled to G Subunits of Heterotrimeric G12-Protein to Regulate Gene Transcription and Neuronal Morphology

Elena Kvachnina,^1 * Guoquan Liu,^2 * Alexander Dityatev,³ Ute Renner,¹ Aline Dumuis,⁴ Diethelm W. Richter,¹ Galina Dityateva,³ Melitta Schachner,³ Tatyana A. Voyno-Yasenetskaya,² and Evgeni G. Ponimaskin¹

¹Abteilung Neurologie und Sinnesphysiologie, Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37073 Göttingen, Germany, ²Department of Pharmacology, University of Illinois, Chicago, Illinois 60612, ³Zentrum für Molekulare Neurobiologie, University of Hamburg, 20246 Hamburg, Germany, and ⁴ Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Universités Montpellier I and II, Montpellier F34094, France

The neurotransmitter serotonin (5-HT) plays an important role in the regulation of multiple events in the CNS. We demonstrated recently a coupling between the 5-HT₄ receptor and the heterotrimeric G13-protein resulting in RhoA-dependent neurite retraction and cell rounding (Ponimaskin et al., 2002). In the present study, we identified G12 as an additional G-protein that can be activated by another member of serotonin receptors, the 5-HT₇ receptor. Expression of 5-HT₇ receptor induced constitutive and agonist-dependent activation of a serum response element-mediated gene transcription through G12-mediated activation of small GTPases. In NIH3T3 cells, activation of the 5-HT₇ receptor induced filopodia formation via a Cdc42-mediated pathway correlating with RhoA-dependent cell rounding. In mouse hippocampal neurons, activation of the endogenous 5-HT₇ receptors significantly increased neurite length, whereas stimulation of 5-HT₄ receptors led to a decrease in the length and number of neurites. These data demonstrate distinct roles for 5-HT₇R/G12 and 5-HT₄R/G13 signaling pathways in neurite outgrowth and retraction, suggesting that serotonin plays a prominent role in regulating the neuronal cytoarchitecture in addition to its classical role as neurotransmitter.

Key words: 5-HT; serotonin; G-protein-coupled receptor; heterotrimeric G-protein; small GTPases; gene transcription; neuronal morphology

---

Received Jan 6, 2005; revised July 7, 2005; accepted July 8, 2005.

This article has been cited by other articles:

				J. A. Knight, C. Smith, N. Toohey, M. T. Klein, and M. Teitler Pharmacological Analysis of the Novel, Rapid, and Potent Inactivation of the Human 5-Hydroxytryptamine7 Receptor by Risperidone, 9-OH-Risperidone, and Other Inactivating Antagonists Mol. Pharmacol., February 1, 2009; 75(2): 374 - 380. [Abstract] [Full Text] [PDF]

				V. P. Pai and N. D. Horseman Biphasic Regulation of Mammary Epithelial Resistance by Serotonin through Activation of Multiple Pathways J. Biol. Chem., November 7, 2008; 283(45): 30901 - 30910. [Abstract] [Full Text] [PDF]

				E. Ponimaskin, G. Dityateva, M. O. Ruonala, M. Fukata, Y. Fukata, F. Kobe, F. S. Wouters, M. Delling, D. S. Bredt, M. Schachner, et al. Fibroblast Growth Factor-Regulated Palmitoylation of the Neural Cell Adhesion Molecule Determines Neuronal Morphogenesis J. Neurosci., September 3, 2008; 28(36): 8897 - 8907. [Abstract] [Full Text] [PDF]

				S. Thakker-Varia, J. J. Krol, J. Nettleton, P. M. Bilimoria, D. A. Bangasser, T. J. Shors, I. B. Black, and J. Alder The Neuropeptide VGF Produces Antidepressant-Like Behavioral Effects and Enhances Proliferation in the Hippocampus J. Neurosci., November 7, 2007; 27(45): 12156 - 12167. [Abstract] [Full Text] [PDF]

				M. A. Stull, V. Pai, A. J. Vomachka, A. M. Marshall, G. A. Jacob, and N. D. Horseman Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions PNAS, October 16, 2007; 104(42): 16708 - 16713. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help