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The Journal of Neuroscience, September 28, 2005, 25(39):8938-8947; doi:10.1523/JNEUROSCI.2415-05.2005
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Behavioral/Systems/Cognitive
Regulation of Ventral Surface Chemoreceptors by the Central Respiratory Pattern Generator
Patrice G. Guyenet, Daniel K. Mulkey, Ruth L. Stornetta, andDouglas A. Bayliss Department of Pharmacology, University of Virginia, Charlottesville, Virginia 22908
The rat retrotrapezoid nucleus (RTN) contains neurons described as central chemoreceptors in the adult and respiratory rhythm-generating pacemakers in neonates [parafacial respiratory group (pfRG)]. Here we test the hypothesis that both RTN and pfRG neurons are intrinsically chemosensitive and tonically firing neurons whose respiratory rhythmicity is caused by a synaptic feedback from the central respiratory pattern generator (CPG). In halothane-anesthetized adults, RTN neurons were silent below 4.5% end-expiratory (e-exp) CO2. Their activity increased linearly (3.2 Hz/1% CO2) up to 6.5% (CPG threshold) and then more slowly to peak
10 Hz at 10% CO2. Respiratory modulation of RTN neurons was absent below CPG threshold, gradually stronger beyond, and, like pfRG neurons, typically (42%) characterized by twin periods of reduced activity near phrenic inspiration. After CPG inactivation with kynurenate (KYN), RTN neurons discharged linearly as a function of e-exp CO2 (slope, +1.7 Hz/1% CO2) and arterial pH (threshold, 7.48; slope, 39 Hz/pH unit). In coronal brain slices (postnatal days 7–12), RTN chemosensitive neurons were silent at pH 7.55. Their activity increased linearly with acidification up to pH 7.2 (17 Hz/pH unit at 35°C) and was always tonic.
In conclusion, consistent with their postulated central chemoreceptor role, RTN/pfRG neurons encode pH linearly and discharge tonically when disconnected from the rest of the respiratory centers in vivo (KYN treatment) and in vitro. In vivo, RTN neurons receive respiratory synchronous inhibitory inputs that may serve as feedback and impart these neurons with their characteristic respiratory modulation.
Key words: central chemoreceptors; medulla oblongata; ventral medullary surface; parafacial respiratory group; dendritic structure; respiratory modulation
Received June 13, 2005; revised July 28, 2005; accepted August 16, 2005.
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