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The Journal of Neuroscience, January 26, 2005, 25(4):843-847; doi:10.1523/JNEUROSCI.4446-04.2005

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BRIEF COMMUNICATION
Mouse Taste Buds Use Serotonin as a Neurotransmitter

Yi-Jen Huang,¹ Yutaka Maruyama,¹ Kuo-Shyan Lu,¹ Elizabeth Pereira,¹ Ilya Plonsky,¹ John E. Baur,¹ Dianqing Wu,¹ and Stephen D. Roper^1,2

¹Department of Physiology and Biophysics and ²Program in Neuroscience, University of Miami School of Medicine, Miami, Florida 33136

Synapses between gustatory receptor cells and primary sensory afferent fibers transmit the output signal from taste buds to the CNS. Several transmitter candidates have been proposed for these synapses, including serotonin (5-HT), glutamate, acetylcholine, ATP, peptides, and others, but, to date, none has been unambiguously identified. We used Chinese hamster ovary cells stably expressing 5-HT_2C receptors as biodetectors to monitor 5-HT release from taste buds. When taste buds were depolarized with KCl or stimulated with bitter, sweet, or sour (acid) tastants, serotonin was released. KCl- and acid-induced 5-HT release, but not release attributable to sweet or bitter stimulation, required Ca²⁺ influx. In contrast, 5-HT release evoked by sweet and bitter stimulation seemed to be triggered by intracellular Ca²⁺ release. These experiments strongly implicate serotonin as a taste bud neurotransmitter and reveal unexpected transmitter release mechanisms.

Key words: biosensors; taste; serotonin; transmitters; calcium imaging; phospholipase C 2

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Received Oct 27, 2004; revised November 30, 2004; accepted November 30, 2004.

This article has been cited by other articles:

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