QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, October 19, 2005, 25(42):9704-9720; doi:10.1523/JNEUROSCI.1621-05.2005

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Yue, C. Articles by Yaari, Y. Search for Related Content PubMed PubMed Citation Articles by Yue, C. Articles by Yaari, Y.

 Previous Article  |  Next Article 

Cellular/Molecular
Proximal Persistent Na⁺ Channels Drive Spike Afterdepolarizations and Associated Bursting in Adult CA1 Pyramidal Cells

Cuiyong Yue,^1 * Stefan Remy,^2 * Hailing Su,¹ Heinz Beck,² and Yoel Yaari¹

¹Department of Physiology, Institute of Medical Sciences, Hebrew University-Hadassah Faculty of Medicine, Jerusalem 91120, Israel, and ²Department of Epileptology, University of Bonn Medical Center, D-53105 Bonn, Germany

In many principal brain neurons, the fast, all-or-none Na⁺ spike initiated at the proximal axon is followed by a slow, graded afterdepolarization (ADP). The spike ADP is critically important in determining the firing mode of many neurons; large ADPs cause neurons to fire bursts of spikes rather than solitary spikes. Nonetheless, not much is known about how and where spike ADPs are initiated. We addressed these questions in adult CA1 pyramidal cells, which manifest conspicuous somatic spike ADPs and an associated propensity for bursting, using sharp and patch microelectrode recordings in acutely isolated hippocampal slices and single neurons. Voltage-clamp commands mimicking spike waveforms evoked transient Na⁺ spike currents that declined quickly after the spike but were followed by substantial sustained Na⁺ spike aftercurrents. Drugs that blocked the persistent Na⁺ current (I_NaP), markedly suppressed the sustained Na⁺ spike aftercurrents, as well as spike ADPs and associated bursting. Ca²⁺ spike aftercurrents were much smaller, and reducing them had no noticeable effect on the spike ADPs. Truncating the apical dendrites affected neither spike ADPs nor the firing modes of these neurons. Application of I_NaP blockers to truncated neurons, or their focal application to the somatic region of intact neurons, suppressed spike ADPs and associated bursting, whereas their focal application to distal dendrites did not. We conclude that the somatic spike ADPs are generated predominantly by persistent Na⁺ channels located at or near the soma. Through this action, proximal I_NaP critically determines the firing mode and spike output of adult CA1 pyramidal cells.

Key words: burst; hippocampus; phenytoin; persistent sodium current; afterdepolarization; M current; PKC; CA1 pyramidal cell; riluzole

---

Received June 22, 2004; revised September 6, 2005; accepted September 6, 2005.

This article has been cited by other articles:

				M. M. Shah, M. Migliore, I. Valencia, E. C. Cooper, and D. A. Brown Functional significance of axonal Kv7 channels in hippocampal pyramidal neurons PNAS, June 3, 2008; 105(22): 7869 - 7874. [Abstract] [Full Text] [PDF]

				S. Chen and Y. Yaari Spike Ca2+ influx upmodulates the spike afterdepolarization and bursting via intracellular inhibition of KV7/M channels J. Physiol., March 1, 2008; 586(5): 1351 - 1363. [Abstract] [Full Text] [PDF]

				M. Yoshida and A. Alonso Cell-Type Specific Modulation of Intrinsic Firing Properties and Subthreshold Membrane Oscillations by the M(Kv7)-Current in Neurons of the Entorhinal Cortex J Neurophysiol, November 1, 2007; 98(5): 2779 - 2794. [Abstract] [Full Text] [PDF]

				E. Chiang and B. W. Strowbridge Diversity of Neural Signals Mediated by Multiple, Burst-Firing Mechanisms in Rat Olfactory Tubercle Neurons J Neurophysiol, November 1, 2007; 98(5): 2716 - 2728. [Abstract] [Full Text] [PDF]

				S. Remy and N. Spruston Dendritic spikes induce single-burst long-term potentiation PNAS, October 23, 2007; 104(43): 17192 - 17197. [Abstract] [Full Text] [PDF]

				L. D. Ellis, W. H. Mehaffey, E. Harvey-Girard, R. W. Turner, L. Maler, and R. J. Dunn SK Channels Provide a Novel Mechanism for the Control of Frequency Tuning in Electrosensory Neurons J. Neurosci., August 29, 2007; 27(35): 9491 - 9502. [Abstract] [Full Text] [PDF]

				A. Reboreda, R. Raouf, A. Alonso, and P. Seguela Development of Cholinergic Modulation and Graded Persistent Activity in Layer V of Medial Entorhinal Cortex J Neurophysiol, June 1, 2007; 97(6): 3937 - 3947. [Abstract] [Full Text] [PDF]

				A. E. Metz, N. Spruston, and M. Martina Dendritic D-type potassium currents inhibit the spike afterdepolarization in rat hippocampal CA1 pyramidal neurons J. Physiol., May 15, 2007; 581(1): 175 - 187. [Abstract] [Full Text] [PDF]

				M. Weiergraber, M. Henry, K. Radhakrishnan, J. Hescheler, and T. Schneider Hippocampal Seizure Resistance and Reduced Neuronal Excitotoxicity in Mice Lacking the Cav2.3 E/R-Type Voltage-Gated Calcium Channel J Neurophysiol, May 1, 2007; 97(5): 3660 - 3669. [Abstract] [Full Text] [PDF]

				Y. Yaari, C. Yue, and H. Su Recruitment of apical dendritic T-type Ca2+ channels by backpropagating spikes underlies de novo intrinsic bursting in hippocampal epileptogenesis J. Physiol., April 15, 2007; 580(2): 435 - 450. [Abstract] [Full Text] [PDF]

				M. W. Bagnall, R. J. Stevens, and S. du Lac Transgenic Mouse Lines Subdivide Medial Vestibular Nucleus Neurons into Discrete, Neurochemically Distinct Populations J. Neurosci., February 28, 2007; 27(9): 2318 - 2330. [Abstract] [Full Text] [PDF]

				Y. Kim and L. O. Trussell Ion Channels Generating Complex Spikes in Cartwheel Cells of the Dorsal Cochlear Nucleus J Neurophysiol, February 1, 2007; 97(2): 1705 - 1725. [Abstract] [Full Text] [PDF]

				A. Peretz, A. Sheinin, C. Yue, N. Degani-Katzav, G. Gibor, R. Nachman, A. Gopin, E. Tam, D. Shabat, Y. Yaari, et al. Pre- and Postsynaptic Activation of M-Channels By a Novel Opener Dampens Neuronal Firing and Transmitter Release J Neurophysiol, January 1, 2007; 97(1): 283 - 295. [Abstract] [Full Text] [PDF]

				F. Brocard, D. Verdier, I. Arsenault, J. P. Lund, and A. Kolta Emergence of Intrinsic Bursting in Trigeminal Sensory Neurons Parallels the Acquisition of Mastication in Weanling Rats J Neurophysiol, November 1, 2006; 96(5): 2410 - 2424. [Abstract] [Full Text] [PDF]

				D. Golomb, C. Yue, and Y. Yaari Contribution of Persistent Na+ Current and M-Type K+ Current to Somatic Bursting in CA1 Pyramidal Cells: Combined Experimental and Modeling Study J Neurophysiol, October 1, 2006; 96(4): 1912 - 1926. [Abstract] [Full Text] [PDF]

				O. Zaika, L. S. Lara, N. Gamper, D. W. Hilgemann, D. B. Jaffe, and M. S. Shapiro Angiotensin II regulates neuronal excitability via phosphatidylinositol 4,5-bisphosphate-dependent modulation of Kv7 (M-type) K+ channels J. Physiol., August 15, 2006; 575(1): 49 - 67. [Abstract] [Full Text] [PDF]

				C. Yue and Y. Yaari Axo-Somatic and Apical Dendritic Kv7/M Channels Differentially Regulate the Intrinsic Excitability of Adult Rat CA1 Pyramidal Cells J Neurophysiol, June 1, 2006; 95(6): 3480 - 3495. [Abstract] [Full Text] [PDF]

				N. Astman, M. J. Gutnick, and I. A. Fleidervish Persistent sodium current in layer 5 neocortical neurons is primarily generated in the proximal axon. J. Neurosci., March 29, 2006; 26(13): 3465 - 3473. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help