QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

The Journal of Neuroscience, November 2, 2005, 25(44):10290-10298; doi:10.1523/JNEUROSCI.3657-05.2005

This Article Full Text Full Text (PDF) Supplemental data Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Koike, C. Articles by Furukawa, T. Search for Related Content PubMed PubMed Citation Articles by Koike, C. Articles by Furukawa, T.

 Previous Article  |  Next Article 

Development/Plasticity/Repair
Function of Atypical Protein Kinase C in Differentiating Photoreceptors Is Required for Proper Lamination of Mouse Retina

Chieko Koike,¹ Akihiro Nishida,¹ Kazunori Akimoto,³ Masa-aki Nakaya,³ Tetsuo Noda,⁴ Shigeo Ohno,³ and Takahisa Furukawa^1,2

¹Department of Developmental Biology, Osaka Bioscience Institute, Osaka 565-0874, Japan, ²Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation, Saitama 332-0012, Japan, ³Department of Molecular Biology, Yokohama City University School of Medicine, Yokohama 239-0004, Japan, and ⁴Department of Cell Biology, Cancer Institute, Tokyo 135-8550, Japan

The photoreceptor is a highly polarized neuron and also has epithelial characteristics such as adherens junctions. To investigate the mechanisms of polarity formation of the photoreceptor cells, we conditionally knocked out atypical protein kinase C (aPKC), which has been proposed to play a critical role in the establishment of epithelial and neuronal polarity, in differentiating photoreceptor cells using the Cre-loxP system. In aPKC conditional knock-out (CKO) mice, the photoreceptor cells displayed morphological defects and failed to form ribbon synapses. Intriguingly, lack of aPKC in differentiating photoreceptors led to severe laminar disorganization not only in the photoreceptor layer but also in the entire retina. Cell fate determination was not affected by total laminar disorganization. After Cre recombinase began to be expressed in the developing photoreceptors at embryonic day 12.5, both the immature photoreceptors and mitotic progenitors were dispersed throughout the CKO retina. We detected that adherens junction formation between the immature photoreceptors and the progenitors was lost in the CKO retina, whereas it was maintained between the progenitors themselves. These results indicate that the expression of aPKC in differentiating photoreceptors is required for total retinal lamination. Our data suggest that properly polarized photoreceptors anchor progenitors at the apical edge of the neural retina, which may be essential for building correct laminar organization of the retina.

Key words: aPKC; retina; photoreceptor; lamina; polarity; adherens junction; cadherin; catenin

---

Received April 27, 2005; revised September 26, 2005; accepted September 28, 2005.

This article has been cited by other articles:

				R. A. Poche, K. M. Kwan, M. A. Raven, Y. Furuta, B. E. Reese, and R. R. Behringer Lim1 Is Essential for the Correct Laminar Positioning of Retinal Horizontal Cells J. Neurosci., December 19, 2007; 27(51): 14099 - 14107. [Abstract] [Full Text] [PDF]

				J. D. Lee, N. F. Silva-Gagliardi, U. Tepass, C. J. McGlade, and K. V. Anderson The FERM protein Epb4.1l5 is required for organization of the neural plate and for the epithelial-mesenchymal transition at the primitive streak of the mouse embryo Development, June 1, 2007; 134(11): 2007 - 2016. [Abstract] [Full Text] [PDF]

				E. Bertrand, C. Fritsch, S. Diether, G. Lambrou, D. Muller, F. Schaeffel, P. Schindler, K. L. Schmid, J. van Oostrum, and H. Voshol Identification of Apolipoprotein A-I as a "STOP" Signal for Myopia Mol. Cell. Proteomics, November 1, 2006; 5(11): 2158 - 2166. [Abstract] [Full Text] [PDF]

				C. Afonso and D. Henrique PAR3 acts as a molecular organizer to define the apical domain of chick neuroepithelial cells J. Cell Sci., October 15, 2006; 119(20): 4293 - 4304. [Abstract] [Full Text] [PDF]

				A. G.S.H. van Rossum, W. M. Aartsen, J. Meuleman, J. Klooster, A. Malysheva, I. Versteeg, J.-P. Arsanto, A. Le Bivic, and J. Wijnholds Pals1/Mpp5 is required for correct localization of Crb1 at the subapical region in polarized Muller glia cells Hum. Mol. Genet., September 15, 2006; 15(18): 2659 - 2672. [Abstract] [Full Text] [PDF]

				F. Imai, S.-i. Hirai, K. Akimoto, H. Koyama, T. Miyata, M. Ogawa, S. Noguchi, T. Sasaoka, T. Noda, and S. Ohno Inactivation of aPKC{lambda} results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex Development, May 1, 2006; 133(9): 1735 - 1744. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help