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The Journal of Neuroscience, March 8, 2006, 26(10):2820-2829; doi:10.1523/JNEUROSCI.5037-05.2006

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Development/Plasticity/Repair
RE-1 Silencer of Transcription/Neural Restrictive Silencer Factor Modulates Ectodermal Patterning during Xenopus Development

Patricio Olguín,¹ Pablo Oteíza,¹ Eduardo Gamboa,¹ José Luis Gómez-Skármeta,² and Manuel Kukuljan¹

¹Centro de Neurociencias Integradas, Iniciativa Científica Milenio, and Programa de Fisiología y Biofísica, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, 838-0453 Independencia, Chile, and ²Centro Andaluz de Biología del Desarrollo, Consejo Superior de Investigaciones Científicas/Universidad Pablo de Olavide, 41013 Sevilla, Spain

Correspondence should be addressed to Manuel Kukuljan and Patricio Olguín, Programa de Fisiología y Biofísica, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Avenida Independencia 1027, 838-0453 Independencia, Chile. Email: kukuljan{at}neuro.med.uchile.cl and polguin{at}med.uchile.cl

RE-1 silencer of transcription/neural restrictive silencer factor (REST/NRSF), a transcriptional repressor, binds to the RE-1 element present in many vertebrate genes. In vitro studies indicate that REST/NRSF plays important roles in several stages of neural development. However, a full understanding of its physiological function requires in vivo approaches. We find that impairment of REST/NRSF function in Xenopus embryos leads to the perturbation of neural tube, cranial ganglia, and eye development. The origin of these defects is the abnormal patterning of the ectoderm during gastrulation. Interference of REST/NRSF function during the late blastula stage leads to an expansion of the neural plate, concomitant with a decrease of the expression of epidermal keratin and neural crest markers. Furthermore, neurogenesis proceeds abnormally, with loss of the expression of proneural, neurogenic, and neuronal genes. The interference of REST/NRSF mimics several features associated with a decreased bone morphogenetic protein (BMP) function and counteracts some effects of BMP4 misexpression. Our results indicate that REST/NRSF function is required in vivo for the acquisition of specific ectodermal cell fates.

Key words: neurogenesis; neural induction; differentiation; transcription factor; ectoderm; neural precursor

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Received Aug. 10, 2005; revised Jan. 20, 2006; accepted Jan. 24, 2006.

Correspondence should be addressed to Manuel Kukuljan and Patricio Olguín, Programa de Fisiología y Biofísica, Instituto de Ciencias Biomedicas, Facultad de Medicina, Universidad de Chile, Avenida Independencia 1027, 838-0453 Independencia, Chile. Email: kukuljan{at}neuro.med.uchile.cl and polguin{at}med.uchile.cl

This article has been cited by other articles:

				L. Ooi, N. D. Belyaev, K. Miyake, I. C. Wood, and N. J. Buckley BRG1 Chromatin Remodeling Activity Is Required for Efficient Chromatin Binding by Repressor Element 1-silencing Transcription Factor (REST) and Facilitates REST-mediated Repression J. Biol. Chem., December 22, 2006; 281(51): 38974 - 38980. [Abstract] [Full Text] [PDF]

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