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The Journal of Neuroscience, March 15, 2006, 26(11):3030-3036; doi:10.1523/JNEUROSCI.5275-05.2006

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Cellular/Molecular
Double Patch Clamp Reveals That Transient Fusion (Kiss-and-Run) Is a Major Mechanism of Secretion in Calf Adrenal Chromaffin Cells: High Calcium Shifts the Mechanism from Kiss-and-Run to Complete Fusion

Abdeladim Elhamdani, Fouad Azizi, and Cristina R. Artalejo

Department of Pharmacology, Wayne State University School of Medicine, Detroit, Michigan 48201

Correspondence should be addressed to Dr. Abdeladim Elhamdani, Department of Pharmacology, Wayne State University, 540 East Canfield Avenue, Detroit, MI 48201. Email: aelhamda{at}med.wayne.edu

Transient fusion ("kiss-and-run") is accepted as a mode of transmitter release both in central neurons and neuroendocrine cells, but the prevalence of this mechanism compared with full fusion is still in doubt. Using a novel double patch-clamp method (whole cell/cell attached), permitting the recording of unitary capacitance events while stimulating under a variety of conditions including action potentials, we show that transient fusion is the predominant (>90%) mode of secretion in calf adrenal chromaffin cells. Raising intracellular Ca²⁺ concentration ([Ca]_i) from 10 to 200 µM increases the incidence of full fusion events at the expense of transient fusion. Blocking rapid endocytosis that normally terminates transient fusion events also promotes full fusion events. Thus, [Ca]_i controls the transition between transient and full fusion, each of which is coupled to different modes of endocytosis.

Key words: exocytosis; endocytosis; kiss-and-run; fusion pore; membrane capacitance; chromaffin cell

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Received Dec. 9, 2005; revised Feb. 1, 2006; accepted Feb. 4, 2006.

Correspondence should be addressed to Dr. Abdeladim Elhamdani, Department of Pharmacology, Wayne State University, 540 East Canfield Avenue, Detroit, MI 48201. Email: aelhamda{at}med.wayne.edu

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